Dark fermentation of hydrogen from waste glycerol using hyperthermophilic eubacterium Thermotoga neapolitana

Tien Ngo Anh, Sang Jun Sim

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Waste glycerol from bio-diesel manufacturing is currently held to be a new, attractive, and abundant resource for future bio-fuel. Bio-hydrogen production via the fermentation route was examined for the first time from pure glycerol and discharged waste glycerol as the sole substrate by the hyperthermophilic eubacterium Thermotoga neapolitana. The bacterium, directly fermenting pure glycerol and crude waste glycerol in the medium, showed respective retarded growth and low H2 production 447 +/- 22 mL H2 L-1 and 437 +/- 21 mL H2 L-1. Attempting to improve the growth and H2 production, small amount (1.5 g L-1) of itaconic acid was added into the culture medium. In this case, H2 production from fermentation was approximately 620 +/- 30 mL H2 L-1 for both pure glycerol and pre-treated waste glycerol as main substrate, an increase of 36.6% compared with cultures grown without itaconic acid, respectively. Further optimization of the glycerol concentration and culture conditions revealed enhancements in growth, H2 production, and glycerol utilization. The H2 fermentation from waste glycerol is of potential importance as wastes from bio-diesel processes can be utilized to produce bio-hydrogen. (c) 2011 American Institute of Chemical Engineers Environ Prog, 2011
Original languageEnglish
JournalEnvironmental Progress & Sustainable Energy
Volume31
Issue number3
Pages (from-to)466-473
Number of pages8
ISSN1944-7442
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • ENGINEERING,
  • ENVIRONMENTAL
  • CALDICELLULOSIRUPTOR-SACCHAROLYTICUS
  • BIODIESEL PRODUCTION
  • CARBON SOURCE
  • GROWTH
  • 1,3-PROPANEDIOL
  • CONVERSION
  • MARITIMA
  • GLUCOSE
  • ETHANOL
  • H-2
  • bio-diesel
  • glycerol
  • hydrogen production
  • Thermotaga neapolitana
  • Water Science and Technology
  • Waste Management and Disposal
  • Environmental Engineering
  • Environmental Chemistry
  • Chemical Engineering (all)
  • Renewable Energy, Sustainability and the Environment
  • Environmental Science (all)
  • Abundant resources
  • Bio-hydrogen
  • Bio-hydrogen production
  • Culture conditions
  • Culture medium
  • Dark fermentation
  • Glycerol concentration
  • Hyperthermophilic
  • Itaconic acid
  • Manufacturing IS
  • Pre-treated wastes
  • Thermotoganeapolitana
  • Fermentation
  • Hydrogen
  • Hydrogen production
  • Substrates
  • Glycerol
  • Bacteria (microorganisms)
  • Thermotoga neapolitana
  • bio‐diesel

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