Danger in the reef: proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis

Andreas Hougaard Laustsen, José Maria Gutiérrez, Arne Redsted Rasmussen, Mikael Engmark, Peter Gravlund, Kate L. Sanders, Brian Lohse, Bruno Lomonte

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    Abstract

    Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF analyses. A. laevis venom is remarkably simple and consists of phospholipases A2 (71.2%), three-finger toxins (3FTx; 25.3%), cysteine-rich secretory proteins (CRISP; 2.5%), and traces of a complement control module protein (CCM; 0.2%). Using a Toxicity Score, the most lethal components were determined to be short neurotoxins. Whole venom had an intravenous LD50 of 0.07 mg/kg in mice and showed a high phospholipase A2 activity, but no proteinase activity in vitro. Preclinical assessment of neutralization and ELISA immunoprofiling showed that BioCSL Sea Snake Antivenom was effective in cross-neutralizing A. laevis venom with an ED50 of 821 μg venom per mL antivenom, with a binding preference towards short neurotoxins, due to the high degree of conservation between short neurotoxins from A. laevisand Enhydrina schistosa venom. Our results point towards the possibility of developing recombinant antibodies or synthetic inhibitors against A. laevis venom due to its simplicity.
    Original languageEnglish
    JournalToxicon
    Volume107
    Issue numberPart B
    Pages (from-to)187-196
    Number of pages10
    ISSN0041-0101
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Aipysurus laevis
    • Olive sea snake
    • Snake venom
    • Proteomics
    • Toxicity
    • Venomics

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