TY - JOUR
T1 - Daily sperm production
T2 - Application in studies of prenatal exposure to nanoparticles in mice
AU - Kyjovska, Zdenka Orabi
AU - Boisen, Anne Mette Zenner
AU - Jackson, Petra
AU - Wallin, Håkan
AU - Vogel, Ulla Birgitte
AU - Hougaard, Karin Sørig
PY - 2013
Y1 - 2013
N2 - We investigated the influence of maternal airway exposure to nanoparticulate titanium dioxide (TiO2, UV-Titan) and carbon black (CB, Printex90), on male reproductive function in the two following generations. Time-mated C57BL/6J mice were exposed by inhalation to UV-Titan, or by intratracheal instillation with Printex90. Body and testicle weight, sperm content per g testicular parenchyma and daily sperm production (DSP) were assessed. The protocol for assessment of DSP was optimized for application in mice (C57BL/6J) and the influence of different parameters was studied. Maternal particulate exposure did not affect DSP statistically significantly in the F1 generation, although TiO2 tended to reduce sperm counts. Overall, time-to-first F2 litter increased with decreasing sperm production. There was no effect on sperm production in the F2 generation originating after TiO2 exposure. F2 offspring, whose fathers were prenatally exposed to Printex90, showed lowered sperm production. Furthermore, we report statistically significant differences in sperm production between mouse strains.
AB - We investigated the influence of maternal airway exposure to nanoparticulate titanium dioxide (TiO2, UV-Titan) and carbon black (CB, Printex90), on male reproductive function in the two following generations. Time-mated C57BL/6J mice were exposed by inhalation to UV-Titan, or by intratracheal instillation with Printex90. Body and testicle weight, sperm content per g testicular parenchyma and daily sperm production (DSP) were assessed. The protocol for assessment of DSP was optimized for application in mice (C57BL/6J) and the influence of different parameters was studied. Maternal particulate exposure did not affect DSP statistically significantly in the F1 generation, although TiO2 tended to reduce sperm counts. Overall, time-to-first F2 litter increased with decreasing sperm production. There was no effect on sperm production in the F2 generation originating after TiO2 exposure. F2 offspring, whose fathers were prenatally exposed to Printex90, showed lowered sperm production. Furthermore, we report statistically significant differences in sperm production between mouse strains.
KW - Developmental toxicity
KW - Prenatal exposure
KW - Male reproduction
KW - Spermatogenesis
KW - Daily sperm production
KW - Nanoparticles
KW - Titanium dioxide
KW - Carbon black
KW - Fertility
KW - Nanotoxicology
U2 - 10.1016/j.reprotox.2012.12.005
DO - 10.1016/j.reprotox.2012.12.005
M3 - Journal article
C2 - 23295323
SN - 0890-6238
VL - 36
SP - 88
EP - 97
JO - Reproductive Toxicology
JF - Reproductive Toxicology
ER -