Cytokine gene expression profiles in chicken spleen and intestinal tissues during Ascaridia galli infection

Janne A. Pleidrup, Liselotte R. Norup, Tina S. Dalgaard, Pete Kaiser, Anders Permin, Torben W. Schou, Dorte Fink Vadekær, Gregers Jungersen, Poul Sørensen, Helle R. Juul-Madsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In the poultry production industry, chickens with access to outdoor areas are exposed to a wide range of parasites e.g. the helminth Ascaridia galli. By real-time quantitative RTPCR, the relative gene expression of the T helper 1 (Th1) cytokine IFN-gamma, the T helper 2 (Th2) cytokine IL-13, the anti-inflammatory cytokines IL-10 and TGF-beta 4 and the proinflammatory cytokine IL-17F were determined over a period of 3 weeks in A. galli and non-A. galli-infected chickens. A characteristic Th2 response was observed in the jejunum of A. galli-infected chickens with increased expression of IL-13 and decreased expression of IFN-gamma from day 14 post infection. At the putative time of larvae invasion into the intestinal mucosa (day 7), an increased expression of IFN-gamma, IL-10, and TGF-beta 4 was observed in the spleen. At the putative onset of the innate immune response (day 10), a decreased expression of jejunal IFN-gamma and IL-13 was observed. Finally, at the expected period of an adaptive immune response (days 14-21) a general decreased expression of IFN-gamma and TGF-beta 4 in spleen and IFN-gamma in jejunum was followed by a decreased expression of IFN-gamma and IL-10 at day 21 in caecal tonsils. (C) 2014 Elsevier B.V. All rights reserved.
Original languageEnglish
JournalVeterinary Parasitology
Volume206
Issue number3-4
Pages (from-to)317-321
Number of pages5
ISSN0304-4017
DOIs
Publication statusPublished - 2014

Keywords

  • Chicken
  • A.galli
  • Immune response
  • Cytokines

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