Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets

L.S. Stevenson, K. McCullough, I. Vincent, D.F. Gilpin, A. Summerfield, Jens Nielsen, F. McNeilly, B.M. Adair, G.M. Allan

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The purpose of this study was to determine serum profiles of cytokines at a protein level and C-reactive protein (CRP) during the development of postweaning multisystemic wasting syndrome (PMWS) in experimentally inoculated pigs. Levels of serum IFN-alpha, IL-6, IL-10, and CRP were examined for a 35-day period in 10 piglets experimentally infected with PCV2 at 3 weeks of age. Four of the infected piglets developed severe PMWS at 14 to 21 days post-infection (d.p.i.) and died prior to termination of the experiment. The remaining six PCV2-infected piglets experienced transient fever, but did not display overt clinical signs of PMWS and were considered as subclinically infected. A bioassay was used to detect IL-6 and ELISAs were used to detect IFN-a, IL-10, and CRIP. There were no significant differences in cytokine or CRP expression from 0 to 7 d.p.i. between the PMWS-affected and the subclinically infected piglets. Levels of IL-10 and CRP were elevated from 10 and 14 d.p.i. respectively in the PMWS-affected piglets compared to the subclinically infected piglets. There were no significant differences in IFN-a and IL-6 expression between the PMWS-affected piglets and the subclinically infected piglets. The present study shows that elevated levels of serum CRP and IL-10 were associated with PCV2-infected piglets that subsequently developed severe PMWS. This may help to provide further insight into the immunoaetiogenesis of this syndrome.
    Original languageEnglish
    JournalViral Immunology
    Volume19
    Issue number2
    Pages (from-to)189-195
    ISSN0882-8245
    DOIs
    Publication statusPublished - 2006

    Cite this

    Stevenson, L. S., McCullough, K., Vincent, I., Gilpin, D. F., Summerfield, A., Nielsen, J., McNeilly, F., Adair, B. M., & Allan, G. M. (2006). Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets. Viral Immunology, 19(2), 189-195. https://doi.org/10.1089/vim.2006.19.189