Cytochrome oxidase as an indicator of ice storage and frozen storage

Helene Godiksen, Flemming Jessen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The potential of cytochrome oxidase as an indicator of ice storage and frozen storage of fish was investigated. Optimal assay conditions for cytochrome oxidase in a crude homogenate from cod muscle were studied. Maximal cytochrome oxidase activity was found at pH 6.5-7.5 and an assay temperature of 30 degreesC. Maximal activation by Triton X-100 was obtained in a range of 0.62-1.25 mM Triton X-100. The specificity of the assay was high, as cytochrome oxidase was inhibited 98% by 33 muM of the specific inhibitor sodium azide. The coefficient of variation of cytochrome oxidase activity in different cods was 21%, and the coefficient of variation of different analyses on the same homogenate was 5%. It was shown that ice storage of muscle samples before they were frozen and thawed resulted in a major freezing-induced activation of cytochrome oxidase activity. The enzyme may therefore be used as an indicator of frozen fish to determine if the fish has been stored on ice before freezing. Cytochrome oxidase activity showed also potential as an indicator of frozen storage, as it was possible to distinguish between the frozen storage temperatures -9, -20, and -40 degreesC.
Original languageEnglish
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number9
Pages (from-to)4488-4493
ISSN0021-8561
DOIs
Publication statusPublished - 2001

Cite this

@article{4d2dfa4f2418418d90c56d82dd1935ec,
title = "Cytochrome oxidase as an indicator of ice storage and frozen storage",
abstract = "The potential of cytochrome oxidase as an indicator of ice storage and frozen storage of fish was investigated. Optimal assay conditions for cytochrome oxidase in a crude homogenate from cod muscle were studied. Maximal cytochrome oxidase activity was found at pH 6.5-7.5 and an assay temperature of 30 degreesC. Maximal activation by Triton X-100 was obtained in a range of 0.62-1.25 mM Triton X-100. The specificity of the assay was high, as cytochrome oxidase was inhibited 98{\%} by 33 muM of the specific inhibitor sodium azide. The coefficient of variation of cytochrome oxidase activity in different cods was 21{\%}, and the coefficient of variation of different analyses on the same homogenate was 5{\%}. It was shown that ice storage of muscle samples before they were frozen and thawed resulted in a major freezing-induced activation of cytochrome oxidase activity. The enzyme may therefore be used as an indicator of frozen fish to determine if the fish has been stored on ice before freezing. Cytochrome oxidase activity showed also potential as an indicator of frozen storage, as it was possible to distinguish between the frozen storage temperatures -9, -20, and -40 degreesC.",
keywords = "R{\aa}vare- og produktteknologi",
author = "Helene Godiksen and Flemming Jessen",
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doi = "10.1021/jf0015219",
language = "English",
volume = "49",
pages = "4488--4493",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
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Cytochrome oxidase as an indicator of ice storage and frozen storage. / Godiksen, Helene; Jessen, Flemming.

In: Journal of Agricultural and Food Chemistry, Vol. 49, No. 9, 2001, p. 4488-4493.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Cytochrome oxidase as an indicator of ice storage and frozen storage

AU - Godiksen, Helene

AU - Jessen, Flemming

PY - 2001

Y1 - 2001

N2 - The potential of cytochrome oxidase as an indicator of ice storage and frozen storage of fish was investigated. Optimal assay conditions for cytochrome oxidase in a crude homogenate from cod muscle were studied. Maximal cytochrome oxidase activity was found at pH 6.5-7.5 and an assay temperature of 30 degreesC. Maximal activation by Triton X-100 was obtained in a range of 0.62-1.25 mM Triton X-100. The specificity of the assay was high, as cytochrome oxidase was inhibited 98% by 33 muM of the specific inhibitor sodium azide. The coefficient of variation of cytochrome oxidase activity in different cods was 21%, and the coefficient of variation of different analyses on the same homogenate was 5%. It was shown that ice storage of muscle samples before they were frozen and thawed resulted in a major freezing-induced activation of cytochrome oxidase activity. The enzyme may therefore be used as an indicator of frozen fish to determine if the fish has been stored on ice before freezing. Cytochrome oxidase activity showed also potential as an indicator of frozen storage, as it was possible to distinguish between the frozen storage temperatures -9, -20, and -40 degreesC.

AB - The potential of cytochrome oxidase as an indicator of ice storage and frozen storage of fish was investigated. Optimal assay conditions for cytochrome oxidase in a crude homogenate from cod muscle were studied. Maximal cytochrome oxidase activity was found at pH 6.5-7.5 and an assay temperature of 30 degreesC. Maximal activation by Triton X-100 was obtained in a range of 0.62-1.25 mM Triton X-100. The specificity of the assay was high, as cytochrome oxidase was inhibited 98% by 33 muM of the specific inhibitor sodium azide. The coefficient of variation of cytochrome oxidase activity in different cods was 21%, and the coefficient of variation of different analyses on the same homogenate was 5%. It was shown that ice storage of muscle samples before they were frozen and thawed resulted in a major freezing-induced activation of cytochrome oxidase activity. The enzyme may therefore be used as an indicator of frozen fish to determine if the fish has been stored on ice before freezing. Cytochrome oxidase activity showed also potential as an indicator of frozen storage, as it was possible to distinguish between the frozen storage temperatures -9, -20, and -40 degreesC.

KW - Råvare- og produktteknologi

U2 - 10.1021/jf0015219

DO - 10.1021/jf0015219

M3 - Journal article

VL - 49

SP - 4488

EP - 4493

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 9

ER -