Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited

Thomas D. Leser, Joanna Zeitman Amenuvor, Tim Kåre Jensen, Rikke H. Lindecrona, Mette Boye, Kristian Møller

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    The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Issue number2
    Pages (from-to)673-690
    Publication statusPublished - 2002

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