Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited

Thomas D. Leser; Joanna Zeitman Amenuvor; Tim Kåre Jensen; Rikke H. Lindecrona; Mette Boye; Kristian Møller

doi:10.1128/AEM.68.2.673-690.2002

Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited

Thomas D. Leser, Joanna Zeitman Amenuvor, Tim Kåre Jensen, Rikke H. Lindecrona, Mette Boye, Kristian Møller

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a

Original language	English
Journal	Applied and Environmental Microbiology
Volume	68
Issue number	2
Pages (from-to)	673-690
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.68.2.673-690.2002
Publication status	Published - 2002

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10.1128/AEM.68.2.673-690.2002

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Leser, T. D., Amenuvor, J. Z., Jensen, T. K., Lindecrona, R. H., Boye, M., & Møller, K. (2002). Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Applied and Environmental Microbiology, 68(2), 673-690. https://doi.org/10.1128/AEM.68.2.673-690.2002

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Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. / Leser, Thomas D.; Amenuvor, Joanna Zeitman; Jensen, Tim Kåre; Lindecrona, Rikke H.; Boye, Mette; Møller, Kristian.

In: Applied and Environmental Microbiology, Vol. 68, No. 2, 2002, p. 673-690.

Research output: Contribution to journal › Journal article › Research › peer-review

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AU - Amenuvor, Joanna Zeitman

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AU - Boye, Mette

AU - Møller, Kristian

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DO - 10.1128/AEM.68.2.673-690.2002

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