TY - JOUR
T1 - Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited
AU - Leser, Thomas D.
AU - Amenuvor, Joanna Zeitman
AU - Jensen, Tim Kåre
AU - Lindecrona, Rikke H.
AU - Boye, Mette
AU - Møller, Kristian
PY - 2002
Y1 - 2002
N2 - The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a
AB - The phylogenetic diversity of the intestinal bacterial community in pigs was studied by comparative 16S ribosomal DNA (rDNA) sequence analysis. Samples were collected from a total of 24 pigs representing a variety of diets, ages, and herd health status. A library comprising 4,270 cloned 16S rDNA sequences obtained directly by PCR from 52 samples of either the ileum, the cecum, or the colon was constructed. In total, 375 phylotypes were identified using a 97% similarity criterion. Three hundred nine of the phylotypes (83%) had a
U2 - 10.1128/AEM.68.2.673-690.2002
DO - 10.1128/AEM.68.2.673-690.2002
M3 - Journal article
VL - 68
SP - 673
EP - 690
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 2
ER -