Cultivar identification by means of isoenzymes 2. Genetic variation at 4 enzyme loci in diploid ryegrass lolium-spp

G. Nielsen, H. Østergaard, Henrik Johansen

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    Fifteen cultivars of perennial ryegrass (L. perenne) and 6 cultivars of Italian ryegrass (L. multiflorum) have been scored for four enzyme loci, Pgi-2, Got-3, Pgm-1 and Pgd-1 [phosphoglucoisomerase, glutamate oxalacetate transaminase, phosphoglucomutase and 6-phosphogluconate isomerase] by starch gel electrophoresis. Cultivars were pairwise compared by means of allelic frequencies assuming Hardy-Weinberg proportions. At first, the comparisons were carried out for each locus separately. The 2 loci Pgi-2 and Got-3 distinguished perennial ryegrass cultivars fairly well, but the discrimination in Italian ryegrass was unsatisfactory at any single locus. By summing the test values for each locus, a combined evaluation of distinctness was obtained. Then cultivars could be pairwise distinguished with 99% confidence in 96% of pairs of perennial ryegrass and in 80% of pairs of Italian ryegrass. Indications of instability were found in some cultivars from a few tests of homogeneity of additional seed lots. General biochemical, genetic and statistical prerequisites for the use of isoenzyme variation in cultivar identification and registration are discussed.
    Original languageEnglish
    JournalZeitschrift fuer Pflanzenzuechtung
    Issue number1
    Pages (from-to)74-86
    Publication statusPublished - 1985

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