Crystallization of [Fe4S3]-ferredoxin from the hyperthermophile archaeon pyrococcus furiosus

Michael Ericsson Skovbo Nielsen; Pernille Harris; Hans Erik Mølager Christensen

Crystallization of [Fe4S3]-ferredoxin from the hyperthermophile archaeon pyrococcus furiosus

Michael Ericsson Skovbo Nielsen
, Pernille Harris
, Hans Erik Mølager Christensen

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.

Original language	English
Journal	Acta Crystallographica Section D-Biological Crystallography
Volume	59
Pages (from-to)	2325-2327
ISSN	0907-4449
Publication status	Published - 2003

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@article{caa60945aed24045a268d265d9dc1be0,

title = "Crystallization of [Fe4S3]-ferredoxin from the hyperthermophile archaeon pyrococcus furiosus",

abstract = "Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30\% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.",

author = "Nielsen, \{Michael Ericsson Skovbo\} and Pernille Harris and Christensen, \{Hans Erik M{\o}lager\}",

year = "2003",

language = "English",

volume = "59",

pages = "2325--2327",

journal = "Acta Crystallographica Section D-Biological Crystallography",

issn = "0907-4449",

publisher = "John Wiley \& Sons Inc.",

}

TY - JOUR

T1 - Crystallization of [Fe4S3]-ferredoxin from the hyperthermophile archaeon pyrococcus furiosus

AU - Nielsen, Michael Ericsson Skovbo

AU - Harris, Pernille

AU - Christensen, Hans Erik Mølager

PY - 2003

Y1 - 2003

N2 - Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.

AB - Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.

M3 - Journal article

SN - 0907-4449

VL - 59

SP - 2325

EP - 2327

JO - Acta Crystallographica Section D-Biological Crystallography

JF - Acta Crystallographica Section D-Biological Crystallography

ER -