TY - JOUR
T1 - Crystallization of [Fe4S3]-ferredoxin from the hyperthermophile archaeon pyrococcus furiosus
AU - Nielsen, Michael Ericsson Skovbo
AU - Harris, Pernille
AU - Christensen, Hans Erik Mølager
PY - 2003
Y1 - 2003
N2 - Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.
AB - Recombinant Pyrococcus furiosus ferredoxin with a [Fe3S4]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 Angstrom, beta = 103.86degrees, and diffract to 1.5 Angstrom resolution.
M3 - Journal article
SN - 0907-4449
VL - 59
SP - 2325
EP - 2327
JO - Acta Crystallographica Section D-Biological Crystallography
JF - Acta Crystallographica Section D-Biological Crystallography
ER -