TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of beta-alanine synthase from the yeast Saccharomyces kluyveri
AU - Dobritzsch, D.
AU - Gojkovic, Zoran
AU - Andersen, Birgit
AU - Piskur, Jure
PY - 2003
Y1 - 2003
N2 - In eukaryotes and some bacteria, the third step of reductive pyrimidine catabolism is catalyzed by beta-alanine synthase (EC 3.5.1.6). Crystals of the recombinant enzyme from the yeast Saccharomyces kluyveri were obtained using sodium citrate as a precipitant. The crystals belong to space group P2(1) (unit-cell parameters a=117.2, b=77.1, c=225.5 Angstrom, beta=95.0degrees) and contain four homodimers per asymmetric unit. Data were collected to 2.7 Angstrom resolution. Introduction of heavy atoms into the crystal lattice induced a different set of unit-cell parameters (a=61.0, b=77.9, c=110.1 Angstrom, beta=97.2degrees) in the same space group P2(1), with only one homodimer per asymmetric unit.
AB - In eukaryotes and some bacteria, the third step of reductive pyrimidine catabolism is catalyzed by beta-alanine synthase (EC 3.5.1.6). Crystals of the recombinant enzyme from the yeast Saccharomyces kluyveri were obtained using sodium citrate as a precipitant. The crystals belong to space group P2(1) (unit-cell parameters a=117.2, b=77.1, c=225.5 Angstrom, beta=95.0degrees) and contain four homodimers per asymmetric unit. Data were collected to 2.7 Angstrom resolution. Introduction of heavy atoms into the crystal lattice induced a different set of unit-cell parameters (a=61.0, b=77.9, c=110.1 Angstrom, beta=97.2degrees) in the same space group P2(1), with only one homodimer per asymmetric unit.
KW - analysis
U2 - 10.1107/S0907444903009120
DO - 10.1107/S0907444903009120
M3 - Journal article
SN - 0907-4449
VL - 59
SP - 1267
EP - 1269
JO - Acta Crystallographica Section D-Biological Crystallography
JF - Acta Crystallographica Section D-Biological Crystallography
ER -