Critical review on biofilm methods

Joana Azeredo, Nuno F. Azevedo, Romain Briandet, Nuno Cerca, Tom Coenye, Ana Rita Costa, Mickaël Desvaux, Giovanni Di Bonaventura, Michel Hébraud, Zoran Jaglic, Miroslava Kačániová, Susanne Knøchel, Anália Lourenço, Filipe Mergulhão, Rikke Louise Meyer, George Nychas, Manuel Simões, Claus Sternberg

Research output: Contribution to journalReviewResearchpeer-review

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Abstract

Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms.
Original languageEnglish
JournalCritical Reviews in Microbiology
Volume43
Issue number3
Pages (from-to)313-351
ISSN1040-841X
DOIs
Publication statusPublished - 2017

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/bync-nd/4.0/), which permits non- commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

Keywords

  • Biofilm formation
  • Biofilm measurement
  • Biofilm data analysis
  • Biofilm omics
  • Biofilm formation devices

Cite this

Azeredo, J., F. Azevedo, N., Briandet, R., Cerca, N., Coenye, T., Rita Costa, A., ... Sternberg, C. (2017). Critical review on biofilm methods. Critical Reviews in Microbiology, 43(3), 313-351. https://doi.org/10.1080/1040841X.2016.1208146
Azeredo, Joana ; F. Azevedo, Nuno ; Briandet, Romain ; Cerca, Nuno ; Coenye, Tom ; Rita Costa, Ana ; Desvaux, Mickaël ; Di Bonaventura, Giovanni ; Hébraud, Michel ; Jaglic, Zoran ; Kačániová, Miroslava ; Knøchel, Susanne ; Lourenço, Anália ; Mergulhão, Filipe ; Meyer, Rikke Louise ; Nychas, George ; Simões, Manuel ; Sternberg, Claus. / Critical review on biofilm methods. In: Critical Reviews in Microbiology. 2017 ; Vol. 43, No. 3. pp. 313-351.
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abstract = "Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms.",
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note = "This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/bync-nd/4.0/), which permits non- commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.",
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Azeredo, J, F. Azevedo, N, Briandet, R, Cerca, N, Coenye, T, Rita Costa, A, Desvaux, M, Di Bonaventura, G, Hébraud, M, Jaglic, Z, Kačániová, M, Knøchel, S, Lourenço, A, Mergulhão, F, Meyer, RL, Nychas, G, Simões, M & Sternberg, C 2017, 'Critical review on biofilm methods', Critical Reviews in Microbiology, vol. 43, no. 3, pp. 313-351. https://doi.org/10.1080/1040841X.2016.1208146

Critical review on biofilm methods. / Azeredo, Joana; F. Azevedo, Nuno; Briandet, Romain; Cerca, Nuno; Coenye, Tom; Rita Costa, Ana; Desvaux, Mickaël; Di Bonaventura, Giovanni; Hébraud, Michel; Jaglic, Zoran; Kačániová, Miroslava; Knøchel, Susanne; Lourenço, Anália; Mergulhão, Filipe; Meyer, Rikke Louise; Nychas, George; Simões, Manuel; Sternberg, Claus.

In: Critical Reviews in Microbiology, Vol. 43, No. 3, 2017, p. 313-351.

Research output: Contribution to journalReviewResearchpeer-review

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T1 - Critical review on biofilm methods

AU - Azeredo, Joana

AU - F. Azevedo, Nuno

AU - Briandet, Romain

AU - Cerca, Nuno

AU - Coenye, Tom

AU - Rita Costa, Ana

AU - Desvaux, Mickaël

AU - Di Bonaventura, Giovanni

AU - Hébraud, Michel

AU - Jaglic, Zoran

AU - Kačániová, Miroslava

AU - Knøchel, Susanne

AU - Lourenço, Anália

AU - Mergulhão, Filipe

AU - Meyer, Rikke Louise

AU - Nychas, George

AU - Simões, Manuel

AU - Sternberg, Claus

N1 - This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/bync-nd/4.0/), which permits non- commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

PY - 2017

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N2 - Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or have a negative impact particularly when formed in industrial settings or on medical devices. As such, research into the formation and elimination of biofilms is important for many disciplines. Several new methodologies have been recently developed for, or adapted to, biofilm studies that have contributed to deeper knowledge on biofilm physiology, structure and composition. In this review, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among the diversity of techniques used to grow and study biofilms. This review intends to remedy this, by giving a critical perspective, highlighting the advantages and limitations of several methods. Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-date methods to study their biofilms.

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KW - Biofilm measurement

KW - Biofilm data analysis

KW - Biofilm omics

KW - Biofilm formation devices

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Azeredo J, F. Azevedo N, Briandet R, Cerca N, Coenye T, Rita Costa A et al. Critical review on biofilm methods. Critical Reviews in Microbiology. 2017;43(3):313-351. https://doi.org/10.1080/1040841X.2016.1208146