CRISPy-web: An online resource to design sgRNAs for CRISPR applications

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Abstract

CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology, allowing the targeted engineering of a wide range of genomes. The system originally evolved in prokaryotes as an adaptive immune system against bacteriophage infections. It now sees widespread application in genome engineering workflows, especially using the Streptococcus pyogenes endonuclease Cas9. To utilize Cas9, so-called single guide RNAs (sgRNAs) need to be designed for each target gene. While there are many tools available to design sgRNAs for the popular model organisms, only few tools that allow designing sgRNAs for non-model organisms exist. Here, we present CRISPy-web (http://crispy.secondarymetabolites.org/), an easy to use web tool based on CRISPy to design sgRNAs for any user-provided microbial genome. CRISPy-web allows researchers to interactively select a region of their genome of interest to scan for possible sgRNAs. After checks for potential off-target matches, the resulting sgRNA sequences are displayed graphically and can be exported to text files. All steps and information are accessible from a web browser without the requirement to install and use command line scripts.
Original languageEnglish
JournalSynthetic and Systems Biotechnology
Volume1
Issue number2
Pages (from-to)118-121
Number of pages4
ISSN2405-805x
DOIs
Publication statusPublished - 2016

Bibliographical note

Open Access funded by KeAi Communications Co. Under a Creative Commons license.

Keywords

  • CRISPR
  • Cas9
  • sgRNA
  • Guide RNA
  • Genome editing
  • Genome engineering

Activities

  • 2 Conference presentations
  • 1 Journal editor

The next generation of 'omics-based natural products discovery

Tilmann Weber (Invited speaker)

20 Jan 2017

Activity: Talks and presentationsConference presentations

In silico and CRISPR/Cas9-based tools for the metabolic engineering of actinomycetes

Tilmann Weber (Invited speaker)

27 Sep 2016

Activity: Talks and presentationsConference presentations

Synthetic and Systems Biotechnology (Journal)

Tilmann Weber (Editor)

Jun 2016

Activity: Editorial work and peer reviewJournal editorResearch

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