Abstract
Interference with genes is the foundation of reverse genetics and is key to manipulation of living cells for biomedical and biotechnological applications. However, classical genetic knockout and transcriptional knockdown technologies have different drawbacks and offer no control over existing protein levels. Here, we describe an efficient genome editing approach that affects specific protein abundances by changing the rates of both RNA synthesis and protein degradation, based on the two cross-kingdom control mechanisms CRISPRi and the N-end rule for protein stability. In addition, our approach demonstrates that CRISPRi efficiency is dependent on endogenous gene expression levels. The method has broad applications in e.g. study of essential genes and antibiotics discovery.
| Original language | English |
|---|---|
| Article number | gkx797 |
| Journal | Nucleic Acids Research |
| Volume | 45 |
| Issue number | 20 |
| Number of pages | 8 |
| ISSN | 0305-1048 |
| DOIs | |
| Publication status | Published - 2017 |
Bibliographical note
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comCite this
Martinez, V., Lauritsen, I., Hobel, T., Li, S., Nielsen, A. T., & Nørholm, M. (2017). CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability. Nucleic Acids Research, 45(20), [gkx797]. https://doi.org/10.1093/nar/gkx797