CRISPR/CAS9 based engineering of actinomycetal genomes

Tilmann Weber (Inventor), Yaojun Tong (Inventor), Sang Yup Lee (Inventor)

Research output: Patent

501 Downloads (Pure)


The present invention relates to CRISPR/Cas-based methods for generating random-sized deletions around at least one target nucleic acid sequence, or for generating precise indels around at least one target nucleic acid sequence, or for modulating transcription of at least one target nucleic acid sequence. Also disclosed is a clonal library comprising clones with random-sized deletions, as well as polynucleotides, polypeptides, cells and kits useful for performing the present methods. The present methods can be performed in organisms where gene editing is typically considered as difficult, such as actinomycetes, in particular streptomycetes.

Original languageEnglish
IPCC12N 15/ 90 A I
Patent numberWO2016150855
Filing date29/09/2016
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date20/03/2015
Priority numberEP20150160126
Publication statusPublished - 29 Sep 2016

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