CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells

Daria Sergeeva, Jose Manuel Camacho Zaragoza, Jae Seong Lee, Helene Faustrup Kildegaard

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

The emergence of CRISPR/Cas9 system as a precise and affordable method for genome editing has prompted its rapid adoption for the targeted integration of transgenes in Chinese hamster ovary (CHO) cells. Targeted gene integration allows the generation of stable cell lines with a controlled and predictable behavior, which is an important feature for the rational design of cell factories aimed at the large-scale production of recombinant proteins. Here we present the protocol for CRISPR/Cas9-mediated integration of a gene expression cassette into a specific genomic locus in CHO cells using homology-directed DNA repair.
Original languageEnglish
Title of host publicationCRISPR Gene Editing
Volume1961
Publication date2019
Pages213-232
Chapter13
DOIs
Publication statusPublished - 2019
SeriesMethods in Molecular Biology
Volume1961
ISSN1064-3745

Keywords

  • CRISPR/Cas9
  • Chinese hamster ovary cells
  • Genome editing
  • Targeted integration

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