CRISPR/Cas system for yeast genome engineering: advances and applications

Vratislav Stovicek; Carina Holkenbrink; Irina Borodina

doi:10.1093/femsyr/fox030

CRISPR/Cas system for yeast genome engineering: advances and applications

Vratislav Stovicek
, Carina Holkenbrink
, Irina Borodina

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.

Original language	English
Journal	F E M S Yeast Research
Volume	17
Issue number	5
Number of pages	16
ISSN	1567-1356
DOIs	https://doi.org/10.1093/femsyr/fox030
Publication status	Published - 2017

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

CRISPR/Cas
CRISPR interference
Genome editing
Yeasts
CRISPR transcriptional regulation
Saccharomyces cerevisiae

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10.1093/femsyr/fox030

Fox030Accepted author manuscript, 1.83 MB
Fox030Final published version, 2.06 MB

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abstract = "The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.",

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AB - The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.

KW - CRISPR/Cas

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CRISPR/Cas system for yeast genome engineering: advances and applications

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