CRISPR/Cas system for yeast genome engineering: advances and applications

Vratislav Stovicek, Carina Holkenbrink, Irina Borodina

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Abstract

The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.
Original languageEnglish
JournalF E M S Yeast Research
Volume17
Issue number5
Number of pages16
ISSN1567-1356
DOIs
Publication statusPublished - 2017

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • CRISPR/Cas
  • CRISPR interference
  • Genome editing
  • Yeasts
  • CRISPR transcriptional regulation
  • Saccharomyces cerevisiae

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