CRISPR/Cas-based genome engineering in natural product discovery

Yaojun Tong, Tilmann Weber, Sang Yup Lee*

*Corresponding author for this work

Research output: Contribution to journalReviewResearchpeer-review

Abstract

This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) – CRISPR/Cas system and how it was engineered to become one of the most important and versatile genome editing techniques that are currently revolutionizing the whole field of molecular biology. It aims to highlight and discuss the applications and remaining challenges of CRISPR/Cas (mainly focusing on CRISPR/SpCas9)-based genome editing in natural product discovery. The organisms covered include bacteria such as Streptomyces, Corynebacteria, and Myxobacteria; filamentous fungi such as Aspergillus, Beauveria, and Ganoderma; microalgae; and some plants. As closing remarks, the prospects of using CRISPR/Cas in natural product discovery will be discussed.
Original languageEnglish
JournalNatural Product Reports
Volume36
Issue number9
Pages (from-to)1225-1372
ISSN0265-0568
DOIs
Publication statusPublished - 2019

Cite this

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title = "CRISPR/Cas-based genome engineering in natural product discovery",
abstract = "This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) – CRISPR/Cas system and how it was engineered to become one of the most important and versatile genome editing techniques that are currently revolutionizing the whole field of molecular biology. It aims to highlight and discuss the applications and remaining challenges of CRISPR/Cas (mainly focusing on CRISPR/SpCas9)-based genome editing in natural product discovery. The organisms covered include bacteria such as Streptomyces, Corynebacteria, and Myxobacteria; filamentous fungi such as Aspergillus, Beauveria, and Ganoderma; microalgae; and some plants. As closing remarks, the prospects of using CRISPR/Cas in natural product discovery will be discussed.",
author = "Yaojun Tong and Tilmann Weber and Lee, {Sang Yup}",
year = "2019",
doi = "10.1039/C8NP00089A",
language = "English",
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journal = "Natural Product Reports",
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publisher = "Royal Society of Chemistry",
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}

CRISPR/Cas-based genome engineering in natural product discovery. / Tong, Yaojun; Weber, Tilmann; Lee, Sang Yup.

In: Natural Product Reports, Vol. 36, No. 9, 2019, p. 1225-1372.

Research output: Contribution to journalReviewResearchpeer-review

TY - JOUR

T1 - CRISPR/Cas-based genome engineering in natural product discovery

AU - Tong, Yaojun

AU - Weber, Tilmann

AU - Lee, Sang Yup

PY - 2019

Y1 - 2019

N2 - This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) – CRISPR/Cas system and how it was engineered to become one of the most important and versatile genome editing techniques that are currently revolutionizing the whole field of molecular biology. It aims to highlight and discuss the applications and remaining challenges of CRISPR/Cas (mainly focusing on CRISPR/SpCas9)-based genome editing in natural product discovery. The organisms covered include bacteria such as Streptomyces, Corynebacteria, and Myxobacteria; filamentous fungi such as Aspergillus, Beauveria, and Ganoderma; microalgae; and some plants. As closing remarks, the prospects of using CRISPR/Cas in natural product discovery will be discussed.

AB - This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) – CRISPR/Cas system and how it was engineered to become one of the most important and versatile genome editing techniques that are currently revolutionizing the whole field of molecular biology. It aims to highlight and discuss the applications and remaining challenges of CRISPR/Cas (mainly focusing on CRISPR/SpCas9)-based genome editing in natural product discovery. The organisms covered include bacteria such as Streptomyces, Corynebacteria, and Myxobacteria; filamentous fungi such as Aspergillus, Beauveria, and Ganoderma; microalgae; and some plants. As closing remarks, the prospects of using CRISPR/Cas in natural product discovery will be discussed.

U2 - 10.1039/C8NP00089A

DO - 10.1039/C8NP00089A

M3 - Review

VL - 36

SP - 1225

EP - 1372

JO - Natural Product Reports

JF - Natural Product Reports

SN - 0265-0568

IS - 9

ER -