CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces

Yongjae Lee, Soonkyu Hwang, Woori Kim, Ji Hun Kim, Bernhard O. Palsson, Byung Kwan Cho*

*Corresponding author for this work

Research output: Contribution to journalReviewpeer-review

Abstract

The demand for discovering novel microbial secondary metabolites is growing to address the limitations in bioactivities such as antibacterial, antifungal, anticancer, anthelmintic, and immunosuppressive functions. Among microbes, the genus Streptomyces holds particular significance for secondary metabolite discovery. Each Streptomyces species typically encodes approximately 30 secondary metabolite biosynthetic gene clusters (smBGCs) within its genome, which are mostly uncharacterized in terms of their products and bioactivities. The development of next-generation sequencing has enabled the identification of a large number of potent smBGCs for novel secondary metabolites that are imbalanced in number compared with discovered secondary metabolites. The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system has revolutionized the translation of enormous genomic potential into the discovery of secondary metabolites as the most efficient genetic engineering tool for Streptomyces. In this review, the current status of CRISPR/Cas applications in Streptomyces is summarized, with particular focus on the identification of secondary metabolite biosynthesis gene clusters and their potential applications. This review summarizes the broad range of CRISPR/Cas applications in Streptomyces for natural product discovery and production. One-Sentence Summary: This review summarizes the broad range of CRISPR/Cas applications in Streptomyces for natural product discovery and production.

Original languageEnglish
Article numberkuae009
JournalJournal of Industrial Microbiology and Biotechnology
Volume51
ISSN1367-5435
DOIs
Publication statusPublished - 2024

Keywords

  • Biosynthetic gene cluster
  • CRISPR/Cas
  • Streptomyces

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