Abstract
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Original language | English |
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Article number | e2007050 |
Journal | PLOS Biology |
Volume | 17 |
Issue number | 3 |
ISSN | 1544-9173 |
DOIs | |
Publication status | Published - 2019 |