186 Downloads (Pure)

Abstract

We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Original languageEnglish
Article numbere2007050
JournalPLOS Biology
Volume17
Issue number3
ISSN1544-9173
DOIs
Publication statusPublished - 2019

Cite this

@article{6a9c812dc6ba4140be9ff4a5d2422b06,
title = "Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses",
abstract = "We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.",
author = "Hao Luo and Hansen, {Anne Sofie L{\ae}rke} and Lei Yang and Konstantin Schneider and Mette Kristensen and Ulla Christensen and Christensen, {Hanne Bjerre} and Bin Du and Emre {\"O}zdemir and Feist, {Adam M} and Keasling, {Jay D} and Jensen, {Michael Krogh} and Herrg{\aa}rd, {Markus J} and Palsson, {Bernhard O}",
year = "2019",
doi = "10.1371/journal.pbio.2007050",
language = "English",
volume = "17",
journal = "P L o S Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses

AU - Luo, Hao

AU - Hansen, Anne Sofie Lærke

AU - Yang, Lei

AU - Schneider, Konstantin

AU - Kristensen, Mette

AU - Christensen, Ulla

AU - Christensen, Hanne Bjerre

AU - Du, Bin

AU - Özdemir, Emre

AU - Feist, Adam M

AU - Keasling, Jay D

AU - Jensen, Michael Krogh

AU - Herrgård, Markus J

AU - Palsson, Bernhard O

PY - 2019

Y1 - 2019

N2 - We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.

AB - We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.

U2 - 10.1371/journal.pbio.2007050

DO - 10.1371/journal.pbio.2007050

M3 - Journal article

VL - 17

JO - P L o S Biology

JF - P L o S Biology

SN - 1544-9173

IS - 3

M1 - e2007050

ER -