We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Luo, H., Hansen, A. S. L., Yang, L.
, Schneider, K., Kristensen, M., Christensen, U., ... Palsson, B. O.
(2019). Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses
. PLOS Biology
(3), [e2007050]. https://doi.org/10.1371/journal.pbio.2007050