Cortisol levels and expression of selected stress- and apoptosis-related genes in the embryos of Atlantic cod, Gadus morhua following short-term exposure to air

Christopher Marlowe, A. Caipang, Ferdinand F. Fagutao, Effrosyni Fatira, Carlo Cabacang Lazado, Michail Pavlidis

Research output: Contribution to journalJournal articleResearchpeer-review

314 Downloads (Pure)

Abstract

Embryos (morula stage) of Atlantic cod, Gadus morhua L., were collected and subjected to air exposure for 2 min. followed by recovery at ambient conditions in the rearing container. Total immunoreactive cortisol and transcription of selected stress- and apoptosis-related genes of the embryos were determined
before the application of the stressor and at 0.5, 1 and 24 h post-exposure. There was no significant difference in the total cortisol levels of the fertilized eggs before and after handling stress. There was high expression level of hsp70 and sod before application of the stressor and significantly increased at 0.5 h postexposure. The expression levels of cat and gpx were weak to moderate and were not affected by the stressor. The apoptotic genes, mcl1 and NR-13 were highly expressed and significantly increased after exposure to air.
Bcl-X1 and Bcl-X2 were moderately expressed in the control samples, but only the expression level of Bcl-X1significantly increased following exposure to air. Cluster analysis of the different gene expression levels indicated three categories: those genes that did not show any change in the expression levels post-air exposure; those that had low expression level in the control followed by a significant increase after air exposure; and those that had high expression levels in the control followed by a further increase in expression after air
exposure. These results clearly demonstrate that there are potential molecular biomarkers of the response in cod embryos as a consequence of air exposure at a time when cortisol is not fully active
Original languageEnglish
JournalInternational Aquatic Research
Volume7
Issue number1
Pages (from-to)75-84
ISSN2008-4935
DOIs
Publication statusPublished - 2015

Bibliographical note

© The Author(s) 2015. This article is published with open access at Springerlink.com

Fingerprint

Dive into the research topics of 'Cortisol levels and expression of selected stress- and apoptosis-related genes in the embryos of Atlantic cod, Gadus morhua following short-term exposure to air'. Together they form a unique fingerprint.

Cite this