Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

Vibeke Frøkjær Jensen, Lotte Jakobsen, Hanne-Dorthe Emborg, Anne Mette Seyfarth, Anette Marie Hammerum

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Objectives: Resistance towards the veterinary drug apramycin can be caused by the aac(3)-IV gene, which also confers resistance towards the important human antibiotic gentamicin. The objectives of this study were to investigate the temporal occurrence and the genetic background of apramycin and gentamicin resistance in Escherichia coli strains from pork, healthy pigs and diagnostic submissions from pigs and to investigate potential relationships to the use of apramycin and gentamicin at farm and national levels. Methods: Data on Danish E. coli isolates from healthy pigs (indicator bacteria), diagnostic submissions from pigs (clinical isolates) and pork were obtained from the national surveillance of antimicrobial resistance and from routine diagnostic laboratories. Antimicrobial consumption data were obtained from the Danish Medicines Agency (1997-2000) and from the VetStat database (2001-2004). The genetic background for gentamicin resistance was investigated by PCR. Relationships between antimicrobial usage and resistance were analysed by chi(2) test and logistic regression. Results: At the farm level, the occurrence of apramycin/gentamicin cross-resistance was correlated to the use of apramycin (P <0.001). At the national level, occurrence of apramycin/gentamicin cross-resistance in clinical E. coli 0149 isolates was significantly correlated with the amounts and duration of apramycin use. The aac(3)-IV gene was detected in all tested cross-resistant isolates. Conclusions: Apramycin consumption at farm level is most probably driving the increasing occurrence of apramycin/gentamicin cross-resistant [aac(3)-IV positive] E. coli in diseased pigs and healthy finishers at slaughter. The duration of use and amounts used both had a significant effect on the prevalence of apramycin/gentamicin cross-resistance in diseased weaning pigs at the national level.
Original languageEnglish
JournalJournal of Antimicrobial Chemotherapy
Volume58
Issue number1
Pages (from-to)101-107
ISSN0305-7453
DOIs
Publication statusPublished - 2006

Cite this

@article{cbd27e6d67944abc9f5ec3350b69e59b,
title = "Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli",
abstract = "Objectives: Resistance towards the veterinary drug apramycin can be caused by the aac(3)-IV gene, which also confers resistance towards the important human antibiotic gentamicin. The objectives of this study were to investigate the temporal occurrence and the genetic background of apramycin and gentamicin resistance in Escherichia coli strains from pork, healthy pigs and diagnostic submissions from pigs and to investigate potential relationships to the use of apramycin and gentamicin at farm and national levels. Methods: Data on Danish E. coli isolates from healthy pigs (indicator bacteria), diagnostic submissions from pigs (clinical isolates) and pork were obtained from the national surveillance of antimicrobial resistance and from routine diagnostic laboratories. Antimicrobial consumption data were obtained from the Danish Medicines Agency (1997-2000) and from the VetStat database (2001-2004). The genetic background for gentamicin resistance was investigated by PCR. Relationships between antimicrobial usage and resistance were analysed by chi(2) test and logistic regression. Results: At the farm level, the occurrence of apramycin/gentamicin cross-resistance was correlated to the use of apramycin (P <0.001). At the national level, occurrence of apramycin/gentamicin cross-resistance in clinical E. coli 0149 isolates was significantly correlated with the amounts and duration of apramycin use. The aac(3)-IV gene was detected in all tested cross-resistant isolates. Conclusions: Apramycin consumption at farm level is most probably driving the increasing occurrence of apramycin/gentamicin cross-resistant [aac(3)-IV positive] E. coli in diseased pigs and healthy finishers at slaughter. The duration of use and amounts used both had a significant effect on the prevalence of apramycin/gentamicin cross-resistance in diseased weaning pigs at the national level.",
author = "Jensen, {Vibeke Fr{\o}kj{\ae}r} and Lotte Jakobsen and Hanne-Dorthe Emborg and Seyfarth, {Anne Mette} and Hammerum, {Anette Marie}",
year = "2006",
doi = "10.1093/jac/dkl201",
language = "English",
volume = "58",
pages = "101--107",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "1",

}

Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli. / Jensen, Vibeke Frøkjær; Jakobsen, Lotte; Emborg, Hanne-Dorthe; Seyfarth, Anne Mette; Hammerum, Anette Marie.

In: Journal of Antimicrobial Chemotherapy, Vol. 58, No. 1, 2006, p. 101-107.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Correlation between apramycin and gentamicin use in pigs and an increasing reservoir of gentamicin-resistant Escherichia coli

AU - Jensen, Vibeke Frøkjær

AU - Jakobsen, Lotte

AU - Emborg, Hanne-Dorthe

AU - Seyfarth, Anne Mette

AU - Hammerum, Anette Marie

PY - 2006

Y1 - 2006

N2 - Objectives: Resistance towards the veterinary drug apramycin can be caused by the aac(3)-IV gene, which also confers resistance towards the important human antibiotic gentamicin. The objectives of this study were to investigate the temporal occurrence and the genetic background of apramycin and gentamicin resistance in Escherichia coli strains from pork, healthy pigs and diagnostic submissions from pigs and to investigate potential relationships to the use of apramycin and gentamicin at farm and national levels. Methods: Data on Danish E. coli isolates from healthy pigs (indicator bacteria), diagnostic submissions from pigs (clinical isolates) and pork were obtained from the national surveillance of antimicrobial resistance and from routine diagnostic laboratories. Antimicrobial consumption data were obtained from the Danish Medicines Agency (1997-2000) and from the VetStat database (2001-2004). The genetic background for gentamicin resistance was investigated by PCR. Relationships between antimicrobial usage and resistance were analysed by chi(2) test and logistic regression. Results: At the farm level, the occurrence of apramycin/gentamicin cross-resistance was correlated to the use of apramycin (P <0.001). At the national level, occurrence of apramycin/gentamicin cross-resistance in clinical E. coli 0149 isolates was significantly correlated with the amounts and duration of apramycin use. The aac(3)-IV gene was detected in all tested cross-resistant isolates. Conclusions: Apramycin consumption at farm level is most probably driving the increasing occurrence of apramycin/gentamicin cross-resistant [aac(3)-IV positive] E. coli in diseased pigs and healthy finishers at slaughter. The duration of use and amounts used both had a significant effect on the prevalence of apramycin/gentamicin cross-resistance in diseased weaning pigs at the national level.

AB - Objectives: Resistance towards the veterinary drug apramycin can be caused by the aac(3)-IV gene, which also confers resistance towards the important human antibiotic gentamicin. The objectives of this study were to investigate the temporal occurrence and the genetic background of apramycin and gentamicin resistance in Escherichia coli strains from pork, healthy pigs and diagnostic submissions from pigs and to investigate potential relationships to the use of apramycin and gentamicin at farm and national levels. Methods: Data on Danish E. coli isolates from healthy pigs (indicator bacteria), diagnostic submissions from pigs (clinical isolates) and pork were obtained from the national surveillance of antimicrobial resistance and from routine diagnostic laboratories. Antimicrobial consumption data were obtained from the Danish Medicines Agency (1997-2000) and from the VetStat database (2001-2004). The genetic background for gentamicin resistance was investigated by PCR. Relationships between antimicrobial usage and resistance were analysed by chi(2) test and logistic regression. Results: At the farm level, the occurrence of apramycin/gentamicin cross-resistance was correlated to the use of apramycin (P <0.001). At the national level, occurrence of apramycin/gentamicin cross-resistance in clinical E. coli 0149 isolates was significantly correlated with the amounts and duration of apramycin use. The aac(3)-IV gene was detected in all tested cross-resistant isolates. Conclusions: Apramycin consumption at farm level is most probably driving the increasing occurrence of apramycin/gentamicin cross-resistant [aac(3)-IV positive] E. coli in diseased pigs and healthy finishers at slaughter. The duration of use and amounts used both had a significant effect on the prevalence of apramycin/gentamicin cross-resistance in diseased weaning pigs at the national level.

U2 - 10.1093/jac/dkl201

DO - 10.1093/jac/dkl201

M3 - Journal article

C2 - 16709594

VL - 58

SP - 101

EP - 107

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 1

ER -