Copper-induced stimulation of nitrification in biological rapid sand filters for drinking water production by proliferation of Nitrosomonas spp.

Florian Benedikt Wagner*, Vaibhav Diwan, Arnaud Dechesne, Jane Fowler, Barth F. Smets, Hans-Jørgen Albrechtsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Copper is a co-factor of the ammonia monooxygenase, an essential enzyme for the activity of ammonia oxidizing prokaryotes (AOP). Copper dosing at less than 1 µg/L stimulated ammonium removal in the poorly-nitrifying biological filters of three full-scale drinking water treatment plants. Upon copper dosing, the ammonium concentration in the effluent decreased from up to 0.18 to less than 0.01 mg NH4+-N/L. To investigate how copper dosing affected the filter microbial community, we applied amplicon sequencing and qPCR targeting key nitrifying groups, including complete ammonia oxidizing (comammox) Nitrospira. Copper dosing increased the abundance of different nitrifiers. Multiple Nitrosomonas variants (betaproteobacterial ammonia oxidizers), which initially collectively represented 1% or less of the total community, increased almost 10 fold. Comammox Nitrospira were abundant and increased too, but their relative abundance within the AOP decreased because of Nitrosomonas proliferation. No other consistent change in the filter communities was detected, as well as no adverse effect of copper on the filters functionality. Our results show that copper dosing in three independent plants was associated with consistent growth of AOP and that efficient nitrification was achieved through the joint contribution of comammox Nitrospira and an increasing fraction of betaproteobacterial ammonia oxidizers.
Original languageEnglish
JournalEnvironmental Science and Technology
Volume53
Issue number21
Pages (from-to)12433-12441
ISSN0013-936X
DOIs
Publication statusPublished - 2019

Cite this

@article{5f19f9992900429b84bde1ea76dd2120,
title = "Copper-induced stimulation of nitrification in biological rapid sand filters for drinking water production by proliferation of Nitrosomonas spp.",
abstract = "Copper is a co-factor of the ammonia monooxygenase, an essential enzyme for the activity of ammonia oxidizing prokaryotes (AOP). Copper dosing at less than 1 µg/L stimulated ammonium removal in the poorly-nitrifying biological filters of three full-scale drinking water treatment plants. Upon copper dosing, the ammonium concentration in the effluent decreased from up to 0.18 to less than 0.01 mg NH4+-N/L. To investigate how copper dosing affected the filter microbial community, we applied amplicon sequencing and qPCR targeting key nitrifying groups, including complete ammonia oxidizing (comammox) Nitrospira. Copper dosing increased the abundance of different nitrifiers. Multiple Nitrosomonas variants (betaproteobacterial ammonia oxidizers), which initially collectively represented 1{\%} or less of the total community, increased almost 10 fold. Comammox Nitrospira were abundant and increased too, but their relative abundance within the AOP decreased because of Nitrosomonas proliferation. No other consistent change in the filter communities was detected, as well as no adverse effect of copper on the filters functionality. Our results show that copper dosing in three independent plants was associated with consistent growth of AOP and that efficient nitrification was achieved through the joint contribution of comammox Nitrospira and an increasing fraction of betaproteobacterial ammonia oxidizers.",
author = "Wagner, {Florian Benedikt} and Vaibhav Diwan and Arnaud Dechesne and Jane Fowler and Smets, {Barth F.} and Hans-J{\o}rgen Albrechtsen",
year = "2019",
doi = "10.1021/acs.est.9b03885",
language = "English",
volume = "53",
pages = "12433--12441",
journal = "Environmental Science & Technology (Washington)",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "21",

}

Copper-induced stimulation of nitrification in biological rapid sand filters for drinking water production by proliferation of Nitrosomonas spp. / Wagner, Florian Benedikt; Diwan, Vaibhav; Dechesne, Arnaud; Fowler, Jane; Smets, Barth F.; Albrechtsen, Hans-Jørgen.

In: Environmental Science and Technology, Vol. 53, No. 21, 2019, p. 12433-12441.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Copper-induced stimulation of nitrification in biological rapid sand filters for drinking water production by proliferation of Nitrosomonas spp.

AU - Wagner, Florian Benedikt

AU - Diwan, Vaibhav

AU - Dechesne, Arnaud

AU - Fowler, Jane

AU - Smets, Barth F.

AU - Albrechtsen, Hans-Jørgen

PY - 2019

Y1 - 2019

N2 - Copper is a co-factor of the ammonia monooxygenase, an essential enzyme for the activity of ammonia oxidizing prokaryotes (AOP). Copper dosing at less than 1 µg/L stimulated ammonium removal in the poorly-nitrifying biological filters of three full-scale drinking water treatment plants. Upon copper dosing, the ammonium concentration in the effluent decreased from up to 0.18 to less than 0.01 mg NH4+-N/L. To investigate how copper dosing affected the filter microbial community, we applied amplicon sequencing and qPCR targeting key nitrifying groups, including complete ammonia oxidizing (comammox) Nitrospira. Copper dosing increased the abundance of different nitrifiers. Multiple Nitrosomonas variants (betaproteobacterial ammonia oxidizers), which initially collectively represented 1% or less of the total community, increased almost 10 fold. Comammox Nitrospira were abundant and increased too, but their relative abundance within the AOP decreased because of Nitrosomonas proliferation. No other consistent change in the filter communities was detected, as well as no adverse effect of copper on the filters functionality. Our results show that copper dosing in three independent plants was associated with consistent growth of AOP and that efficient nitrification was achieved through the joint contribution of comammox Nitrospira and an increasing fraction of betaproteobacterial ammonia oxidizers.

AB - Copper is a co-factor of the ammonia monooxygenase, an essential enzyme for the activity of ammonia oxidizing prokaryotes (AOP). Copper dosing at less than 1 µg/L stimulated ammonium removal in the poorly-nitrifying biological filters of three full-scale drinking water treatment plants. Upon copper dosing, the ammonium concentration in the effluent decreased from up to 0.18 to less than 0.01 mg NH4+-N/L. To investigate how copper dosing affected the filter microbial community, we applied amplicon sequencing and qPCR targeting key nitrifying groups, including complete ammonia oxidizing (comammox) Nitrospira. Copper dosing increased the abundance of different nitrifiers. Multiple Nitrosomonas variants (betaproteobacterial ammonia oxidizers), which initially collectively represented 1% or less of the total community, increased almost 10 fold. Comammox Nitrospira were abundant and increased too, but their relative abundance within the AOP decreased because of Nitrosomonas proliferation. No other consistent change in the filter communities was detected, as well as no adverse effect of copper on the filters functionality. Our results show that copper dosing in three independent plants was associated with consistent growth of AOP and that efficient nitrification was achieved through the joint contribution of comammox Nitrospira and an increasing fraction of betaproteobacterial ammonia oxidizers.

U2 - 10.1021/acs.est.9b03885

DO - 10.1021/acs.est.9b03885

M3 - Journal article

VL - 53

SP - 12433

EP - 12441

JO - Environmental Science & Technology (Washington)

JF - Environmental Science & Technology (Washington)

SN - 0013-936X

IS - 21

ER -