Conventional Treatment of Glioblastoma Reveals Persistent CD44+ Subpopulations

Johann Mar Gudbergsson*, Esben Christensen, Serhii Kostrikov, Torben Moos, Meg Duroux, Andreas Kjær, Kasper Bendix Johnsen*, Thomas Lars Andresen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Glioblastoma (GBM) is the most frequent and devastating primary tumor of the central nervous system with a median survival of 12 to 15 months after diagnosis. GBM is highly difficult to treat due to its delicate location, inter- and intra-tumoral heterogeneity, and high plasticity in response to treatment. In this study, we intracranially implanted primary GBM cells into mice which underwent conventional GBM treatments, including irradiation, temozolomide, and a combination. We obtained single cell suspensions through a combination of mechanical and enzymatic dissociation of brain tissue and investigated in detail the changes in GBM cells in response to conventional treatments in vivo using multi-color flow cytometry and cluster analysis. CD44 expression was elevated in all treatment groups, which was confirmed by subsequent immunohistochemistry. High CD44 expression was furthermore shown to correlate with poor prognosis of GBM and low-grade glioma (LGG) patients. Together, these results indicate a key role for CD44 in glioma pathogenesis.
Original languageEnglish
JournalMolecular Neurobiology
Volume57
Pages (from-to)3943–3955
ISSN0893-7648
DOIs
Publication statusPublished - 2020

Keywords

  • Glioblastoma
  • Cancer
  • Tumor
  • Stem cells
  • CD44
  • CD133
  • Plasticity
  • Treatment
  • Resistance
  • Population

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