Controlling intercellular flow through mechanosensitive plasmodesmata nanopores

Keunhwan Park, Jan Knoblauch, Karl Oparka, Kaare H. Jensen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In plants, plasmodesmata (PD) are nanopores that serve as channels for molecular cell-to-cell transport. Precise control of PD permeability is essential to regulate processes such as growth and tissue patterning, photoassimilate distribution and defense against pathogens. Callose deposition modulates PD transport but little is known of the rapid events that lead to PD closure in response to tissue damage or osmotic shock. We propose a mechanism of PD closure as a result of mechanosensing. Pressure forces acting on the dumbbell-shaped ER-desmotubule complex cause it to be displaced from its equilibrium position, thus closing the PD aperture. The filamentous protein tethers that link the plasma membrane to the ER-desmotubule complex play a key role in determining the selectivity of the PD pore. This model of PD control compares favorably with experimental data on the pressure-generated closure of PD.
Original languageEnglish
Article number3564
JournalNature Communications
Volume10
Issue number1
Number of pages7
ISSN2041-1723
DOIs
Publication statusPublished - 2019

Cite this

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title = "Controlling intercellular flow through mechanosensitive plasmodesmata nanopores",
abstract = "In plants, plasmodesmata (PD) are nanopores that serve as channels for molecular cell-to-cell transport. Precise control of PD permeability is essential to regulate processes such as growth and tissue patterning, photoassimilate distribution and defense against pathogens. Callose deposition modulates PD transport but little is known of the rapid events that lead to PD closure in response to tissue damage or osmotic shock. We propose a mechanism of PD closure as a result of mechanosensing. Pressure forces acting on the dumbbell-shaped ER-desmotubule complex cause it to be displaced from its equilibrium position, thus closing the PD aperture. The filamentous protein tethers that link the plasma membrane to the ER-desmotubule complex play a key role in determining the selectivity of the PD pore. This model of PD control compares favorably with experimental data on the pressure-generated closure of PD.",
author = "Keunhwan Park and Jan Knoblauch and Karl Oparka and Jensen, {Kaare H.}",
year = "2019",
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Controlling intercellular flow through mechanosensitive plasmodesmata nanopores. / Park, Keunhwan; Knoblauch, Jan; Oparka, Karl; Jensen, Kaare H.

In: Nature Communications, Vol. 10, No. 1, 3564, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Controlling intercellular flow through mechanosensitive plasmodesmata nanopores

AU - Park, Keunhwan

AU - Knoblauch, Jan

AU - Oparka, Karl

AU - Jensen, Kaare H.

PY - 2019

Y1 - 2019

N2 - In plants, plasmodesmata (PD) are nanopores that serve as channels for molecular cell-to-cell transport. Precise control of PD permeability is essential to regulate processes such as growth and tissue patterning, photoassimilate distribution and defense against pathogens. Callose deposition modulates PD transport but little is known of the rapid events that lead to PD closure in response to tissue damage or osmotic shock. We propose a mechanism of PD closure as a result of mechanosensing. Pressure forces acting on the dumbbell-shaped ER-desmotubule complex cause it to be displaced from its equilibrium position, thus closing the PD aperture. The filamentous protein tethers that link the plasma membrane to the ER-desmotubule complex play a key role in determining the selectivity of the PD pore. This model of PD control compares favorably with experimental data on the pressure-generated closure of PD.

AB - In plants, plasmodesmata (PD) are nanopores that serve as channels for molecular cell-to-cell transport. Precise control of PD permeability is essential to regulate processes such as growth and tissue patterning, photoassimilate distribution and defense against pathogens. Callose deposition modulates PD transport but little is known of the rapid events that lead to PD closure in response to tissue damage or osmotic shock. We propose a mechanism of PD closure as a result of mechanosensing. Pressure forces acting on the dumbbell-shaped ER-desmotubule complex cause it to be displaced from its equilibrium position, thus closing the PD aperture. The filamentous protein tethers that link the plasma membrane to the ER-desmotubule complex play a key role in determining the selectivity of the PD pore. This model of PD control compares favorably with experimental data on the pressure-generated closure of PD.

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