Controlling Cellular Uptake by Surface Chemistry, Size, and Surface Topology at the Nanoscale

Marzia Massignani, Caterina LoPresti, Adam Blanazs, Peter Jeppe Madsen, Steven P. Armes, Andrew L. Lewis, Giuseppe Battaglia

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Cell cytosol and the different subcellular organelles house the most important biochemical processes that control cell functions. Effective delivery of bioactive agents within cells is expected to have an enormous impact on both gene therapy and the future development of new therapeutic and/or diagnostic strategies based on single-cell-bioactive-agent interactions. Herein a biomimetic nanovector is reported that is able to enter cells, escape from the complex endocytic pathway, and efficiently deliver actives within clinically relevant cells without perturbing their metabolic activity. This nanovector is based on the pH-controlled self-assembly of amphiphilic copolymers into nanometer-sized vesicles (or polymersomes). The cellular-uptake kinetics can be regulated by controlling the surface chemistry, the polymersome size, and the polymersome surface topology. The latter is controlled by the extent of polymer-polymer phase separation within the external envelope of the polymersome.
Original languageEnglish
JournalSmall
Volume5
Issue number21
Pages (from-to)2424-2432
Number of pages9
ISSN1613-6810
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Biomimetics
  • Cells
  • Intracellular delivery
  • Nanovectors
  • Polymersomes

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