Controlled-release biocatalysis for the synthesis of D-Phenylglycine

T. Rojanarata; D. Isarangul; S. Wiyakrutta; V. Meevootisom; John Woodley

doi:10.1080/10242420410001727355

Controlled-release biocatalysis for the synthesis of D-Phenylglycine

T. Rojanarata, D. Isarangul, S. Wiyakrutta, V. Meevootisom, John Woodley

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise d -phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final Dphenylglycine concentration of 10.25 mg/ml.

Original language	English
Journal	Biocatalysis and Biotransformation
Volume	22
Issue number	3
Pages (from-to)	195-201
ISSN	1024-2422
DOIs	https://doi.org/10.1080/10242420410001727355
Publication status	Published - 2004
Externally published	Yes

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title = "Controlled-release biocatalysis for the synthesis of D-Phenylglycine",

abstract = "An isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise d -phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final Dphenylglycine concentration of 10.25 mg/ml. ",

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AU - Isarangul, D.

AU - Wiyakrutta, S.

AU - Meevootisom, V.

AU - Woodley, John

PY - 2004

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AB - An isolated and immobilised aminotransferase cloned from Pseudomonas stutzeri ST-201 into Escherichia coli was used to synthesise d -phenylglycine. The reaction was characterised by an unfavourable equilibrium constant and substrate inhibition. The use of a controlled-release system via the use of Amberlite (IRA 400)-adsorbed benzoylformate proved a useful technique to circumvent these issues. This resulted in a four-fold improvement in product concentration achievable to yield a final Dphenylglycine concentration of 10.25 mg/ml.

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DO - 10.1080/10242420410001727355

M3 - Journal article

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Controlled-release biocatalysis for the synthesis of D-Phenylglycine

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