Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

M.D. Mikkelsen; V.L. Fuller; Bjarne Gram Hansen; M. Nafisi; C.E. Olsen; Henrik Bjørn Nielsen; B.A. Halkier

doi:10.1007/s00425-009-0907-5

Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

M.D. Mikkelsen, V.L. Fuller, Bjarne Gram Hansen, M. Nafisi, C.E. Olsen, Henrik Bjørn Nielsen, B.A. Halkier

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.

Original language	English
Journal	Planta
Volume	229
Issue number	6
Pages (from-to)	1209-1217
ISSN	0032-0935
DOIs	https://doi.org/10.1007/s00425-009-0907-5
Publication status	Published - 2009

Keywords

Indole-3-acetaldoxime
CYP79B2
Camalexin
CYP79B3
Ethanol-inducible
Secondary metabolism
Glucosinolates

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title = "Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2",

abstract = "Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.",

keywords = "Indole-3-acetaldoxime, CYP79B2, Camalexin, CYP79B3, Ethanol-inducible, Secondary metabolism, Glucosinolates",

author = "M.D. Mikkelsen and V.L. Fuller and Hansen, {Bjarne Gram} and M. Nafisi and C.E. Olsen and Nielsen, {Henrik Bj{\o}rn} and B.A. Halkier",

year = "2009",

doi = "10.1007/s00425-009-0907-5",

language = "English",

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pages = "1209--1217",

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T1 - Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

AU - Mikkelsen, M.D.

AU - Fuller, V.L.

AU - Hansen, Bjarne Gram

AU - Nafisi, M.

AU - Olsen, C.E.

AU - Nielsen, Henrik Bjørn

AU - Halkier, B.A.

PY - 2009

Y1 - 2009

N2 - Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.

AB - Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.

KW - Indole-3-acetaldoxime

KW - CYP79B2

KW - Camalexin

KW - CYP79B3

KW - Ethanol-inducible

KW - Secondary metabolism

KW - Glucosinolates

U2 - 10.1007/s00425-009-0907-5

DO - 10.1007/s00425-009-0907-5

M3 - Journal article

VL - 229

SP - 1209

EP - 1217

JO - Planta

JF - Planta

SN - 0032-0935

IS - 6

ER -

Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

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