Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

M.D. Mikkelsen, V.L. Fuller, Bjarne Gram Hansen, M. Nafisi, C.E. Olsen, Henrik Bjørn Nielsen, B.A. Halkier

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
    Original languageEnglish
    JournalPlanta
    Volume229
    Issue number6
    Pages (from-to)1209-1217
    ISSN0032-0935
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Indole-3-acetaldoxime
    • CYP79B2
    • Camalexin
    • CYP79B3
    • Ethanol-inducible
    • Secondary metabolism
    • Glucosinolates

    Cite this

    Mikkelsen, M. D., Fuller, V. L., Hansen, B. G., Nafisi, M., Olsen, C. E., Nielsen, H. B., & Halkier, B. A. (2009). Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2. Planta, 229(6), 1209-1217. https://doi.org/10.1007/s00425-009-0907-5