Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2

M.D. Mikkelsen, V.L. Fuller, Bjarne Gram Hansen, M. Nafisi, C.E. Olsen, Henrik Bjørn Nielsen, B.A. Halkier

    Research output: Contribution to journalJournal articleResearchpeer-review


    Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
    Original languageEnglish
    Issue number6
    Pages (from-to)1209-1217
    Publication statusPublished - 2009


    • Indole-3-acetaldoxime
    • CYP79B2
    • Camalexin
    • CYP79B3
    • Ethanol-inducible
    • Secondary metabolism
    • Glucosinolates


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