Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

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Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid. / Varrone, Cristiano; Floriotis, Georgis; Heggeset, Tonje M. B.; Le, Simone B.; Markussen, Sidsel; Skiadas, Ioannis V; Gavala, Hariklia N.

In: Biochemical Engineering Journal, Vol. 128, 2017, p. 149-161.

Research output: Contribution to journalJournal article – Annual report year: 2017Researchpeer-review

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@article{f769d408bd54480b9e4fbcc099bd662d,
title = "Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid",
abstract = "This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media. The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110 g/L/d of glycerol (with a productivity of 38 g/L/d and 11 g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12 h and 60 g/L feed. The maximum feed concentration reached almost 90 g/L, leading though to an incomplete substrate degradation.",
keywords = "1,3 propanediol, Butyric acid, Crude glycerol, CSTR, Fermentation, Mixed microbial cultures",
author = "Cristiano Varrone and Georgis Floriotis and Heggeset, {Tonje M. B.} and Le, {Simone B.} and Sidsel Markussen and Skiadas, {Ioannis V} and Gavala, {Hariklia N.}",
year = "2017",
doi = "10.1016/j.bej.2017.09.012",
language = "English",
volume = "128",
pages = "149--161",
journal = "Biochemical Engineering Journal",
issn = "1369-703X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Continuous fermentation and kinetic experiments for the conversion of crude glycerol derived from second-generation biodiesel into 1,3 propanediol and butyric acid

AU - Varrone, Cristiano

AU - Floriotis, Georgis

AU - Heggeset, Tonje M. B.

AU - Le, Simone B.

AU - Markussen, Sidsel

AU - Skiadas, Ioannis V

AU - Gavala, Hariklia N.

PY - 2017

Y1 - 2017

N2 - This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media. The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110 g/L/d of glycerol (with a productivity of 38 g/L/d and 11 g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12 h and 60 g/L feed. The maximum feed concentration reached almost 90 g/L, leading though to an incomplete substrate degradation.

AB - This study investigated the performance of different mixed microbial cultures (MMC) able to ferment crude glycerol generated from animal fat-based biodiesel to produce 1,3 propanediol (1,3 PDO) and butyric acid, under non-sterile conditions. Eight different continuous flow stirred-tank reactors (CSTR) were set up with different inoculum types and growth media. The distribution of metabolic products under variable operating conditions was determined. All MMC were characterized from a kinetic point of view and overall stoichiometric reactions were constructed. Changes in the microbial communities were monitored by means of Next Generation Sequencing (NGS). Maximum substrate degradation rate reached approximately 110 g/L/d of glycerol (with a productivity of 38 g/L/d and 11 g/L/d for 1,3 PDO and butyric acid, respectively), obtained with an hydraulic retention time of 12 h and 60 g/L feed. The maximum feed concentration reached almost 90 g/L, leading though to an incomplete substrate degradation.

KW - 1,3 propanediol

KW - Butyric acid

KW - Crude glycerol

KW - CSTR

KW - Fermentation

KW - Mixed microbial cultures

U2 - 10.1016/j.bej.2017.09.012

DO - 10.1016/j.bej.2017.09.012

M3 - Journal article

VL - 128

SP - 149

EP - 161

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

ER -