Continuous Exposure Of Vibrio Anguillarum To Tropodithietic Acid: Genetic Changes And Influence On Virulence

Bastian Barker Rasmussen, Paul D'Alvise, Torben Grotkjær, Mikkel Bentzon-Tilia, Lone Gram

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearchpeer-review

    Abstract

    Introduction: The fish pathogen Vibrio anguillarum is a major problem in aquaculture causing Vibriosis. Bacteria of the Roseobacter clade can antagonize pathogenic vibrios in cultures in live feed such as microalgae, rotifers and Artemia, as well as in fish larvae. Therefore, roseobacters could be promising as probiotics in fish rearing. Production of the antibacterial compound tropodithietic acid (TDA) by roseobacters is key in the antagonism of vibrios. However, the effects of continuous exposure to TDA on V. anguillarum remain unknown. The purpose of this study was to investigate how prolonged TDA exposure affects V. anguillarum focusing on the development of resistance towards TDA and changes in virulence.Methods: Seven lineages of V. anguillarum were exposed to increasing TDA concentrations over 300-400 generations and were subsequently genome sequenced. Virulence of the lineages is currently being tested in fish cell infection trials.Results: Following exposure, four linages reached 1.75 x wild-type MIC and three reached 1.5 x wild-type MIC. Genome sequencing revealed no major changes in the genomes of the lineages. The only virulence-related gene affected was fliM, encoding a flagella motor switch protein. However, mutations in this gene were observed in non-exposed controls as well.Conclusions: In conclusion, TDA resistance does not appear to develop, and the virulence genes of V. anguillarum are unaffected by TDA exposure, supporting the application of TDA-producing roseobacters as probiotics in aquaculture.
    Original languageEnglish
    Title of host publicationThe Danish Microbiological Society Annual Congress 2015 : Programme & Abstracts
    Place of PublicationCopenhagen
    Publication date2015
    Pages72-72
    Publication statusPublished - 2015
    EventThe Danish Microbiological Society Annual Congress 2015 - Eigtved's Pakhus, Copenhagen, Denmark
    Duration: 9 Nov 20159 Nov 2015

    Conference

    ConferenceThe Danish Microbiological Society Annual Congress 2015
    LocationEigtved's Pakhus
    CountryDenmark
    CityCopenhagen
    Period09/11/201509/11/2015

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