Contextualizing context for synthetic biology--identifying causes of failure of synthetic biological systems.

Stefano Cardinale, Adam Paul Arkin

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Despite the efforts that bioengineers have exerted in designing and constructing biological processes that function according to a predetermined set of rules, their operation remains fundamentally circumstantial. The contextual situation in which molecules and single-celled or multicellular organisms find themselves shapes the way they interact, respond to the environment and process external information. Since the birth of the field, synthetic biologists have had to grapple with contextual issues, particularly when the molecular and genetic devices inexplicably fail to function as designed when tested in vivo. In this review, we set out to identify and classify the sources of the unexpected divergences between design and actual function of synthetic systems and analyze possible methodologies aimed at controlling, if not preventing, unwanted contextual issues.
Original languageEnglish
JournalBiotechnology Journal
Volume7
Pages (from-to)856-866
ISSN1860-6768
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Complexity
  • Context
  • Environment
  • Gene expression
  • Synthetic biology

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