Context-Dependent Development of Lymphoid Stroma from Adult CD34+ Adventitial Progenitors

Katarzyna Maria Sitnik, Kerstin Wendland, Holger Weishaupt, Heli Uronen-Hansson, Andrea J. White, Graham Anderson, Knut Kotarsky, William Winston Agace

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    Abstract

    Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3-PDPN+PDGFRβ+/α+CD34+ stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN-PDGFRβ+/α-Esam-1+ITGA7+ pericytes. CD34+ adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34+ adventitial cells are capable of differentiating into multiple lymphoid stroma-like subsets including pericyte-, FRC-, MRC-, and FDC-like cells, the development of which was lymphoid environment-dependent. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34+ adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues.
    Original languageEnglish
    JournalCell Reports
    Volume14
    Issue number10
    Pages (from-to)2375-2388
    Number of pages14
    DOIs
    Publication statusPublished - 2016

    Keywords

    • Biochemistry, Genetics and Molecular Biology (all)

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