Constructs of electrospun PLGA, compressed collagen and minced urothelium for minimally manipulated autologous bladder tissue expansion

Fatemeh Ajalloueian, Said Zeiai, Magdalena Fossum, Jons G. Hilborn

Research output: Contribution to journalJournal articleResearchpeer-review


Bladder regeneration based on minced bladder mucosa in vivo expansion is an alternative to in vitro culturing of urothelial cells. Here, we present the design of a hybrid, electrospun poly(lactic-co-glycolide) (PLGA) - plastically compressed (PC) collagen scaffold that could allow in vivo bladder mucosa expansion. Optimisation of electrospinning was performed in order to obtain increased pore sizes and porosity to consolidate the construct and to support neovascularisation and tissue ingrowth. Tensile tests showed an increase in average tensile strength from 0.6 MPa for PC collagen to 3.57 MPa for the hybrid construct. The optimised PLGA support scaffold was placed between two collagen gels, and the minced tissue was distributed either on top or both on top and inside the construct prior to PC; this was then cultured for up to four weeks. Morphology, histology and SEM demonstrated that the construct maintained its integrity throughout cell culture. Cells from minced tissue migrated, expanded and re-organised to a confluent cell layer on the top of the construct after two weeks and formed a multilayered urothelium after four weeks. Cell morphology and phenotype was typical for urothelial mucosa during tissue culture. (C) 2014 Elsevier Ltd. All rights reserved.
Original languageEnglish
Issue number22
Pages (from-to)5741-5748
Publication statusPublished - 2014
Externally publishedYes


  • Bladder
  • Collagen
  • Electrospinning
  • Minced tissue
  • Plastic compression
  • PLGA
  • Cells
  • Copolymers
  • Cytology
  • Expansion
  • Morphology
  • Tensile strength
  • Tensile testing
  • Tissue
  • Tissue culture
  • Collagen scaffolds
  • Electrospun PLGA
  • Hybrid constructs
  • Tissue in-growth
  • Urothelial cells
  • Scaffolds (biology)
  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics
  • collagen
  • collagen gel
  • cytokeratin
  • mercury
  • polyglactin
  • article
  • bladder mucosa
  • cell culture
  • cell proliferation
  • cell structure
  • electrospinning
  • epithelium cell
  • histology
  • hybrid
  • immunohistochemistry
  • in vivo study
  • neovascularization (pathology)
  • phenotype
  • porosity
  • priority journal
  • scanning electron microscopy
  • staining
  • tensile strength
  • tissue culture
  • tissue expansion
  • tissue growth
  • urothelium
  • Animals
  • Cells, Cultured
  • Gels
  • Lactic Acid
  • Polyglycolic Acid
  • Regeneration
  • Swine
  • Tensile Strength
  • Tissue Engineering
  • Tissue Scaffolds
  • Urinary Bladder
  • Urothelium
  • polylactic acid-polyglycolic acid copolymer
  • 26009-03-0 Polyglycolic Acid
  • 33X04XA5AT Lactic Acid
  • 9007-34-5 Collagen
  • neovascularisation
  • tissue ingrowth
  • Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Suidae [85740] pig common
  • poly(lactic-co-glycolide) PLGA
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10511, Biophysics - Bioengineering
  • 15504, Urinary system - Physiology and biochemistry
  • 32500, Tissue culture, apparatus, methods and media
  • Chemical Coordination and Homeostasis
  • bladder excretory system
  • urothelial mucosa excretory system
  • urothelium excretory system
  • cell culture laboratory techniques, culturing techniques
  • electrospinning laboratory techniques
  • histology laboratory techniques, histology and cytology techniques
  • SEM scanning electron microscopy laboratory techniques, imaging and microscopy techniques
  • Urinary System


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