Construction of Prevotella ruminicola-Escherichia coli shuttle vector pRAM45 and transformation of P. ruminicola strains by electroporation

K. Ogata, Rustam Aminov, K. Tajima, M. Nakamura, H. Matsui, T. Nagamine, Y. Benno

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Abstract

A new plasmid shuttle vector, pRAM45, was constructed from the Escherichia coli plasmid pACYC184 and the cryptic plasmid pRAM4, which was originally isolated by us from Prevofella ruminicola T31 (Ogata et al., Plasmid, 35, 91-97, 1996). The vector was electrotransformed into different P. ruminicola strains. Polymerase chain reaction and Southern hybridization experiments confirmed its integrity in P. ruminicola transformants.
Original languageEnglish
JournalJournal of Bioscience and Bioengineering
Volume88
Issue number3
Pages (from-to)316-318
Number of pages3
ISSN1389-1723
DOIs
Publication statusPublished - 1999
Externally publishedYes

Keywords

  • bacterial DNA
  • plasmid vector
  • article
  • bacterium transformation
  • controlled study
  • electroporation
  • Escherichia coli
  • nonhuman
  • polymerase chain reaction
  • Prevotella ruminicola
  • shuttle vector
  • Southern blotting
  • Bioconversion
  • Enzymes
  • Genetic engineering
  • Electroporation
  • Plasmids
  • Polymerase
  • Southern hybridization
  • Plasmid pRAM4
  • Shuttle vector
  • X
  • plasmid pRAM4
  • BIOTECHNOLOGY
  • FOOD
  • RESTRICTION-ENDONUCLEASE
  • BACTEROIDES-UNIFORMIS
  • PLASMID
  • GENE
  • DNA
  • BACTERIA
  • RUMEN
  • biotechnology
  • pRAM45 shuttle vector construction
  • Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Prevotella ruminicola
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
  • plasmids
  • 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10300, Replication, transcription, translation
  • 10506, Biophysics - Molecular properties and macromolecules
  • 13014, Metabolism - Nucleic acids, purines and pyrimidines
  • 30500, Morphology and cytology of bacteria
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • 32000, Microbiological apparatus, methods and media
  • 39008, Food microbiology - General and miscellaneous
  • Biochemistry and Molecular Biophysics
  • bacterial transformation gene expression/vector techniques, molecular genetic method
  • electroporation gene expression/vector techniques, molecular genetic method
  • Southern blotting Recombinant DNA Technology, analytical method, detection/labeling techniques, gene mapping, molecular probe techniques
  • Molecular Genetics
  • CLONING VECTORS
  • ESCHERICHIA
  • ESCHERICHIA COLI
  • SHUTTLE VECTORS
  • Biotechnology
  • Genetics, molecular biology and recombinant DNA technology

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