Construction of minimal genomes and synthetic cells

Donghui Choe; Sun Chang Kim; Bernhard O. Palsson; Byung-Kwan Cho

doi:10.1007/978-3-030-31897-0_2

Construction of minimal genomes and synthetic cells

Donghui Choe, Sun Chang Kim, Bernhard O. Palsson, Byung-Kwan Cho^*

^*Corresponding author for this work

Novo Nordisk Foundation Center for Biosustainability

Korea Advanced Institute of Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Abstract

A minimal genome strain containing only genes necessary for maintaining self-replicable life was proposed as a potential platform having various advantages in chemical and pharmaceutical industries. With recent advances in high-throughput DNA sequencing and synthesis technology, many reduced genomes have now been constructed. In this chapter, we will review previously constructed artificially reduced genomes to confirm the potential of their industrial utility. Some of them exhibit growth rates similar to those of their parental wild-type strains while offering higher genetic stability and productivity. Furthermore, we will discuss some technological hurdles and limitations encountered during the design and construction of reduced genomes.

Original language	English
Title of host publication	Minimal Cells: Design, Construction, Biotechnological Applications
Editors	Alvaro R. Lara , Guillermo Gosset
Number of pages	23
Publisher	Springer
Publication date	2020
Pages	45-67
ISBN (Print)	978-3-030-31896-3
ISBN (Electronic)	978-3-030-31897-0
DOIs	https://doi.org/10.1007/978-3-030-31897-0_2
Publication status	Published - 2020

Keywords

Genome reduction
Minimal genome
Genome synthesis
Synthetic biology
Adaptive laboratory evolution

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10.1007/978-3-030-31897-0_2

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AU - Choe, Donghui

AU - Kim, Sun Chang

AU - Palsson, Bernhard O.

AU - Cho, Byung-Kwan

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AB - A minimal genome strain containing only genes necessary for maintaining self-replicable life was proposed as a potential platform having various advantages in chemical and pharmaceutical industries. With recent advances in high-throughput DNA sequencing and synthesis technology, many reduced genomes have now been constructed. In this chapter, we will review previously constructed artificially reduced genomes to confirm the potential of their industrial utility. Some of them exhibit growth rates similar to those of their parental wild-type strains while offering higher genetic stability and productivity. Furthermore, we will discuss some technological hurdles and limitations encountered during the design and construction of reduced genomes.

KW - Genome reduction

KW - Minimal genome

KW - Genome synthesis

KW - Synthetic biology

KW - Adaptive laboratory evolution

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BT - Minimal Cells: Design, Construction, Biotechnological Applications

A2 - , Alvaro R. Lara

A2 - , Guillermo Gosset

PB - Springer

ER -

Construction of minimal genomes and synthetic cells

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