Construction of minimal genomes and synthetic cells

Donghui Choe, Sun Chang Kim, Bernhard O. Palsson, Byung-Kwan Cho*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review


A minimal genome strain containing only genes necessary for maintaining self-replicable life was proposed as a potential platform having various advantages in chemical and pharmaceutical industries. With recent advances in high-throughput DNA sequencing and synthesis technology, many reduced genomes have now been constructed. In this chapter, we will review previously constructed artificially reduced genomes to confirm the potential of their industrial utility. Some of them exhibit growth rates similar to those of their parental wild-type strains while offering higher genetic stability and productivity. Furthermore, we will discuss some technological hurdles and limitations encountered during the design and construction of reduced genomes.
Original languageEnglish
Title of host publicationMinimal Cells: Design, Construction, Biotechnological Applications
EditorsAlvaro R. Lara , Guillermo Gosset
Number of pages23
Publication date2020
ISBN (Print)978-3-030-31896-3
ISBN (Electronic)978-3-030-31897-0
Publication statusPublished - 2020


  • Genome reduction
  • Minimal genome
  • Genome synthesis
  • Synthetic biology
  • Adaptive laboratory evolution


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