Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Zhijun Tong; Sanjie Jiang; Weiming He; Xuejun Chen; Lixin Yin; Dunhuang Fang; Yafei Hu; Fangchan Jiao; Chi Zhang; Jianmin Zeng; Xinfu Wu; Shancen Zhao; Jianbo Jian; Bingguang Xiao

doi:10.17221/8/2021-CJGPB

Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Zhijun Tong, Sanjie Jiang, Weiming He, Xuejun Chen, Lixin Yin, Dunhuang Fang, Yafei Hu, Fangchan Jiao, Chi Zhang, Jianmin Zeng, Xinfu Wu, Shancen Zhao, Jianbo Jian^*, Bingguang Xiao^*

^*Corresponding author for this work

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Abstract

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F₁ parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

Original language	English
Journal	Czech Journal of Genetics and Plant Breeding
Volume	57
Issue number	3
Pages (from-to)	102-112
Number of pages	11
ISSN	1212-1975
DOIs	https://doi.org/10.17221/8/2021-CJGPB
Publication status	Published - 2021

Keywords

Crossing
Single nucleotide polymorphism (SNP)
Whole genome sequencing

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Tong, Z., Jiang, S., He, W., Chen, X., Yin, L., Fang, D., Hu, Y., Jiao, F., Zhang, C., Zeng, J., Wu, X., Zhao, S., Jian, J., & Xiao, B. (2021). Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing. Czech Journal of Genetics and Plant Breeding, 57(3), 102-112. https://doi.org/10.17221/8/2021-CJGPB

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title = "Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing",

abstract = "Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.",

keywords = "Crossing, Single nucleotide polymorphism (SNP), Whole genome sequencing",

author = "Zhijun Tong and Sanjie Jiang and Weiming He and Xuejun Chen and Lixin Yin and Dunhuang Fang and Yafei Hu and Fangchan Jiao and Chi Zhang and Jianmin Zeng and Xinfu Wu and Shancen Zhao and Jianbo Jian and Bingguang Xiao",

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doi = "10.17221/8/2021-CJGPB",

language = "English",

volume = "57",

pages = "102--112",

journal = "Czech Journal of Genetics and Plant Breeding",

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Tong, Z, Jiang, S, He, W, Chen, X, Yin, L, Fang, D, Hu, Y, Jiao, F, Zhang, C, Zeng, J, Wu, X, Zhao, S, Jian, J & Xiao, B 2021, 'Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing', Czech Journal of Genetics and Plant Breeding, vol. 57, no. 3, pp. 102-112. https://doi.org/10.17221/8/2021-CJGPB

TY - JOUR

T1 - Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

AU - Tong, Zhijun

AU - Jiang, Sanjie

AU - He, Weiming

AU - Chen, Xuejun

AU - Yin, Lixin

AU - Fang, Dunhuang

AU - Hu, Yafei

AU - Jiao, Fangchan

AU - Zhang, Chi

AU - Zeng, Jianmin

AU - Wu, Xinfu

AU - Zhao, Shancen

AU - Jian, Jianbo

AU - Xiao, Bingguang

PY - 2021

Y1 - 2021

N2 - Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

AB - Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

KW - Crossing

KW - Single nucleotide polymorphism (SNP)

KW - Whole genome sequencing

U2 - 10.17221/8/2021-CJGPB

DO - 10.17221/8/2021-CJGPB

M3 - Journal article

SN - 1212-1975

VL - 57

SP - 102

EP - 112

JO - Czech Journal of Genetics and Plant Breeding

JF - Czech Journal of Genetics and Plant Breeding

IS - 3

ER -

Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

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