Construction of Genetically Marked Ruminococcus-Albus Strains and Conjugal Transfer of Plasmid Pam-Beta-1 into Them

Rustam Aminov, K. Kaneichi, T. Miyagi, K. Sakka, K. Ohmiya

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Abstract

Anaerobic filter matings of Ruminococcus albus F40/2 and A2, both rifampicin- and streptomycin-resistant mutants, were done with Enterococcus faecalis DS16C3, which contains chromosomally inserted Tn 916 and plasmid pAMbeta1, and with Bacillus thuringiensis BT351, which contains pAMbeta1. Erythromycin-resistant transconjugants were obtained in mating of R. albus A2 and B. thuringiensis BT351 with an average frequency of 7 x 10(-8) (per recipient). The identity of plasmid pAMbeta1 in R. albus A3 transconjugants was confirmed by restriction and Southern hybridization analyses.
Original languageEnglish
JournalJournal of fermentation and bioengineering (Print)
Volume78
Issue number1
Pages (from-to)1-5
Number of pages5
ISSN0922-338X
Publication statusPublished - 1994
Externally publishedYes

Keywords

  • FOOD
  • BIOTECHNOLOGY
  • ESCHERICHIA-COLI
  • RESTRICTION ENDONUCLEASE
  • ENTEROCOCCUS-FAECALIS
  • BETA-GLUCOSIDASE
  • CELLULASE GENES
  • DNA
  • RUMEN
  • CLONING
  • ENDO-1,4-BETA-GLUCANASE

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