Abstract
Anaerobic filter matings of Ruminococcus albus F40/2 and A2, both rifampicin- and streptomycin-resistant mutants, were done with Enterococcus faecalis DS16C3, which contains chromosomally inserted Tn 916 and plasmid pAMbeta1, and with Bacillus thuringiensis BT351, which contains pAMbeta1. Erythromycin-resistant transconjugants were obtained in mating of R. albus A2 and B. thuringiensis BT351 with an average frequency of 7 x 10(-8) (per recipient). The identity of plasmid pAMbeta1 in R. albus A3 transconjugants was confirmed by restriction and Southern hybridization analyses.
| Original language | English |
|---|---|
| Journal | Journal of Fermentation and Bioengineering |
| Volume | 78 |
| Issue number | 1 |
| Pages (from-to) | 1-5 |
| Number of pages | 5 |
| ISSN | 0922-338X |
| Publication status | Published - 1994 |
| Externally published | Yes |
Keywords
- FOOD
- BIOTECHNOLOGY
- ESCHERICHIA-COLI
- RESTRICTION ENDONUCLEASE
- ENTEROCOCCUS-FAECALIS
- BETA-GLUCOSIDASE
- CELLULASE GENES
- DNA
- RUMEN
- CLONING
- ENDO-1,4-BETA-GLUCANASE