Abstract
The genomic cleavage map of the type strain Fibrobacter succinogenes S85 was constructed. The restriction enzymes Asci, AvrII, FseI, NofI, and SfiI generated DNA fragments of suitable size distribution that could be resolved by pulsed-field gel electrophoresis (PFGE). An average genome size of 3.6 Mb was obtained by summing the total fragment sizes. The linkages between the 15 AscI fragments of the genome were determined by combining two approaches: isolation of linking clones and crosshybridization of restriction fragments. The genome of F. succinogenes was found to be represented by the single circular DNA molecule. Southern hybridization with specific probes allowed the eight genetic markers to be located on the restriction map. The genome of this bacterium contains at least three rRNA operons. PFGE of the other three strains of F. succinogenes gave estimated genome sizes close to that of the type strain. However, RFLP patterns of these strains generated by Asci digestion are completely different. Pairwise comparison of the genomic fragment distribution between the type strain and the three isolates showed a similarity level in the region of 14.3% to 31.3%. No fragment common to all of these F: succinogenes strains could be detected by PFGE, A marked degree of genomic heterogeneity among members of this species makes genomic III;LP a highly discriminatory and useful molecular typing tool for population studies.
Original language | English |
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Journal | Current Microbiology |
Volume | 35 |
Issue number | 1 |
Pages (from-to) | 22-27 |
Number of pages | 6 |
ISSN | 0343-8651 |
DOIs | |
Publication status | Published - 1997 |
Externally published | Yes |
Keywords
- Animals
- Bacteria
- Cattle
- Chromosome Mapping
- Electrophoresis, Gel, Pulsed-Field
- Female
- Genome, Bacterial
- Rumen
- dna fragment
- restriction endonuclease
- article
- biodiversity
- chromosome rearrangement
- gene mapping
- gene transfer
- genetic linkage
- genetic marker
- genome
- hybridization
- nonhuman
- operon
- priority journal
- pulsed field gel electrophoresis
- restriction fragment
- restriction fragment length polymorphism
- restriction mapping
- southern blotting
- Bacteria (microorganisms)
- Fibrobacter succinogenes
- MICROBIOLOGY
- GENE
- S85
- ENDOGLUCANASE
- SEQUENCE
- ANALYTICAL METHOD
- AVERAGE GENOME SIZE
- DNA
- GENOMIC CLEAVAGE MAP CONSTRUCTION
- GENOMIC DIVERSITY
- GENOMIC FRAGMENT DISTRIBUTION
- MOLECULAR GENETICS
- OPERONS
- PULSED-FIELD GEL ELECTROPHORESIS
- RESTRICTION FRAGMENT LENGTH POLYMORPHISM
- RESTRICTION MAPPING
- RFLP
- SOUTHERN HYBRIDIZATION
- Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Fibrobacter succinogenes
- 10010, Comparative biochemistry
- 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
- 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
- 10300, Replication, transcription, translation
- 10506, Biophysics - Molecular properties and macromolecules
- 14004, Digestive system - Physiology and biochemistry
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- 36002, Medical and clinical microbiology - Bacteriology
- Biochemistry and Molecular Biophysics
- Ingestion and Assimilation
- Digestive System
- Genetics
- Infection
- Molecular Genetics
- Physiology