In this project we have explored the possibility of using designed consortiums for the covalorization of the main carbon sources in lignocellulosic biomass (xylose, glucose, arabinose, and acetic acid). In one study we have used pre processing simulations, constraint-based modelling, and state-of-the art metabolic engineering tools to develop a consortium of cells capable of efficient valorization of synthetic hemicellulosic hydrolysate. Stable co-existence and effective covalorization was achieved through niche-differentiation, auxotrophy, and adaptive evolution. In another study, stable consortia based fermentation was achieved through niche partitioning, syntrophy (auxotrophy combined with removal of inhibitory side product), and CRISPRi mediated gene silencing. The achieved results demonstrate that consortium based approaches for valorizing complex biomass and waste related carbon sources can be an attractive alternative to the design of a so-called “superbug” and can thereby add significant value to biorefineries.
|Number of pages||1|
|Publication status||Published - 2016|
|Event||Sustain-ATV Conference 2016 - Technical University of Denmark, Kgs. Lyngby, Denmark|
Duration: 30 Nov 2016 → 30 Nov 2016
|Conference||Sustain-ATV Conference 2016|
|Location||Technical University of Denmark|
|Period||30/11/2016 → 30/11/2016|