Conformational changes in the solution structure of the dengue virus 5' end in the presence and absence of the 3' untranslated region.

Charlotta Polacek, Jonathan E Foley, Eva Harris

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Dengue virus (DENV) is a approximately 10.7-kb positive-sense RNA virus that circularizes via RNA-RNA interactions between sequences in the 5' and 3' terminal regions. Complementarity between the Cyclization Sequence (CS) and the Upstream AUG Region (UAR) has been shown to be necessary for viral replication. Here, we present the solution structure of the 5' end of DENV type 2 in the presence and absence of the 3' end. We demonstrate hybridization between the 5' and 3' CS that is independent of the UAR, while the 5'-3' UAR hybridization is dependent upon the 5'-3' CS interaction.
Keyword: RNA structure, cyclization, flavivirus, untranslated regions (UTR), RNA-RNA interaction
Original languageEnglish
JournalJournal of Virology
Issue number2
Pages (from-to)1161-1166
Publication statusPublished - 2009
Externally publishedYes

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