Confirmatory analysis of steroids in muscle using liquid chromatography-tandem mass spectrometry

Mikael Pedersen, Jens Hinge Andersen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A method is described for screening and confirmation of synthetic and endogenous steroids in muscle tissue. The method is sensitive, selective, and rapid and the consumption of organic solvents is low, compared to previously published methods. The procedure involves hydrolysis, defattening with heptane and final clean up with SPE using C18 cartridge. After filtration, the analytes are analysed by LC/MS/MS and quantification is performed using deuterated internal standards. Decision limits (CCα) varied from 0.02 to 0.33 μg kg-1 and the detection capabilities (CCβ) were
Original languageEnglish
JournalFood Additives and Contaminants
Volume28
Issue number4
Pages (from-to)428-437
ISSN0265-203X
DOIs
Publication statusPublished - 2011

Fingerprint

Dive into the research topics of 'Confirmatory analysis of steroids in muscle using liquid chromatography-tandem mass spectrometry'. Together they form a unique fingerprint.

Cite this