Concentrating Genomic Length DNA in a Microfabricated Array

Yu Chen, Ezra S. Abrams, T. Christian Boles, Jonas Nyvold Pedersen, Henrik Flyvbjerg, Robert H. Austin, James C. Sturm

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    Abstract

    We demonstrate that a microfabricated bump array can concentrate genomic-length DNA molecules efficiently at continuous, high flow velocities, up to 40 μm=s, if the single-molecule DNA globule has a sufficiently large shear modulus. Increase in the shear modulus is accomplished by compacting the DNA molecules to minimal coil size using polyethylene glycol (PEG) derived depletion forces. We map out the sweet spot, where concentration occurs, as a function of PEG concentration and flow speed using a combination of theoretical analysis and experiment. Purification of DNA from enzymatic reactions for next-generation DNA-sequencing libraries will be an important application of this development.
    Original languageEnglish
    Article number198303
    JournalPhysical Review Letters
    Volume114
    Issue number19
    Number of pages5
    ISSN0031-9007
    DOIs
    Publication statusPublished - 2015

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    © 2015 American Physical Society

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