Concentrated protein solutions investigated using acoustic levitation and small-angle X-ray scattering

Pernille Sønderby, Christopher Söderberg, Christian Grundahl Frankær, Günther H.J. Peters, Jens T Bukrinski, Ana Labrador, Tomás S Plivelic, Pernille Harris*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

An acoustically levitated droplet has been used to collect synchrotron SAXS data on human serum albumin protein solutions up to a protein concentration of 400 mg ml-1. A careful selection of experiments allows for fast data collection of a large amount of data, spanning a protein concentration/solvent concentration space with limited sample consumption (down to 3 µL per experiment) and few measurements. The data analysis shows data of high quality that are reproducible and comparable with data from standard flow-through capillary-based experiments. Furthermore, using this methodology, it is possible to achieve concentrations that would not be accessible by conventional cells. The protein concentration and ionic strength parameter space diagram may be covered easily and the amount of protein sample is significantly reduced (by a factor of 100 in this work). Used in routine measurements, the benefits in terms of protein cost and time spent are very significant.
Original languageEnglish
JournalJournal of Synchrotron Radiation
Volume27
Issue number2
Pages (from-to)396-404
ISSN0909-0495
DOIs
Publication statusPublished - 2020

Keywords

  • SAXS
  • Levitated droplet
  • Concentrated protein solutions
  • Structure factors

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