Computational redesign of a mononuclear zinc metalloenzyme for organophosphate hydrolysis.

Sagar D. Khare, Yakov Kipnis, Per Junior Greisen, Ryo Takeuchi, Yacov Ashani, Moshe Goldsmith, Yifan Song, Jasmine L Gallaher, Israel Silman, Haim Leader, Joel L Sussman, Barry L. Stoddard, Dan S. Tawfik, David Baker

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The ability to redesign enzymes to catalyze noncognate chemical transformations would have wide-ranging applications. We developed a computational method for repurposing the reactivity of metalloenzyme active site functional groups to catalyze new reactions. Using this method, we engineered a zinc-containing mouse adenosine deaminase to catalyze the hydrolysis of a model organophosphate with a catalytic efficiency (k(cat)/K(m)) of ~10(4) M(-1) s(-1) after directed evolution. In the high-resolution crystal structure of the enzyme, all but one of the designed residues adopt the designed conformation. The designed enzyme efficiently catalyzes the hydrolysis of the R(P) isomer of a coumarinyl analog of the nerve agent cyclosarin, and it shows marked substrate selectivity for coumarinyl leaving groups. Computational redesign of native enzyme active sites complements directed evolution methods and offers a general approach for exploring their untapped catalytic potential for new reactivities.
Original languageEnglish
JournalNature Chemical Biology
Volume8
Issue number3
Pages (from-to)294-300
ISSN1552-4450
DOIs
Publication statusPublished - 2012

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