Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications

Fatemeh Ajalloueian*, Nikolaos Nikogeorgos, Ali Ajalloueian, Magdalena Fossum, Seunghwan Lee, Ioannis S. Chronakis

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA. The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved. Nanomechanical characterization of compressed collagen sheets by AFM showed that Young's modulus was inversely proportional to the final concentration of collagen. Similarly, according to SEM, MTS, and cell nuclei counting, all the scaffolds supported cell adhesion and proliferation, whilst the highest metabolic activities and proliferation were seen in the scaffolds with lowest collagen content in hydrogel formula. We conclude that by decreasing the collagen content in the formula of collagen hydrogel for plastic compression, not only a better cell environment and optimum mechanical properties are achieved, but also the application costs of this biopolymer is reduced.
Original languageEnglish
JournalInternational Journal of Biological Macromolecules
Volume108
Pages (from-to)158-166
ISSN0141-8130
DOIs
Publication statusPublished - 2018

Keywords

  • Collagen hydrogel
  • Fibrillar density
  • Fibroblast
  • Infiltration
  • Nanomechanical characteristics
  • Plastic compression
  • Proliferation

Cite this

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title = "Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications",
abstract = "In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA. The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved. Nanomechanical characterization of compressed collagen sheets by AFM showed that Young's modulus was inversely proportional to the final concentration of collagen. Similarly, according to SEM, MTS, and cell nuclei counting, all the scaffolds supported cell adhesion and proliferation, whilst the highest metabolic activities and proliferation were seen in the scaffolds with lowest collagen content in hydrogel formula. We conclude that by decreasing the collagen content in the formula of collagen hydrogel for plastic compression, not only a better cell environment and optimum mechanical properties are achieved, but also the application costs of this biopolymer is reduced.",
keywords = "Collagen hydrogel, Fibrillar density, Fibroblast, Infiltration, Nanomechanical characteristics, Plastic compression, Proliferation",
author = "Fatemeh Ajalloueian and Nikolaos Nikogeorgos and Ali Ajalloueian and Magdalena Fossum and Seunghwan Lee and Chronakis, {Ioannis S.}",
year = "2018",
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language = "English",
volume = "108",
pages = "158--166",
journal = "International Journal of Biological Macromolecules",
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Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications. / Ajalloueian, Fatemeh; Nikogeorgos, Nikolaos; Ajalloueian, Ali; Fossum, Magdalena; Lee, Seunghwan; Chronakis, Ioannis S.

In: International Journal of Biological Macromolecules, Vol. 108, 2018, p. 158-166.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications

AU - Ajalloueian, Fatemeh

AU - Nikogeorgos, Nikolaos

AU - Ajalloueian, Ali

AU - Fossum, Magdalena

AU - Lee, Seunghwan

AU - Chronakis, Ioannis S.

PY - 2018

Y1 - 2018

N2 - In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA. The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved. Nanomechanical characterization of compressed collagen sheets by AFM showed that Young's modulus was inversely proportional to the final concentration of collagen. Similarly, according to SEM, MTS, and cell nuclei counting, all the scaffolds supported cell adhesion and proliferation, whilst the highest metabolic activities and proliferation were seen in the scaffolds with lowest collagen content in hydrogel formula. We conclude that by decreasing the collagen content in the formula of collagen hydrogel for plastic compression, not only a better cell environment and optimum mechanical properties are achieved, but also the application costs of this biopolymer is reduced.

AB - In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA. The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved. Nanomechanical characterization of compressed collagen sheets by AFM showed that Young's modulus was inversely proportional to the final concentration of collagen. Similarly, according to SEM, MTS, and cell nuclei counting, all the scaffolds supported cell adhesion and proliferation, whilst the highest metabolic activities and proliferation were seen in the scaffolds with lowest collagen content in hydrogel formula. We conclude that by decreasing the collagen content in the formula of collagen hydrogel for plastic compression, not only a better cell environment and optimum mechanical properties are achieved, but also the application costs of this biopolymer is reduced.

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