Comprehensive evaluation of different strategies to recover methanogenic performance in ammonia-stressed reactors

Miao Yan, Xinyu Zhu, Laura Treu, Giulia Ravenni, Stefano Campanaro, Estelle Maria Goonesekera, Rosa Ferrigno, Carsten S. Jacobsen, Athanasios Zervas, Irini Angelidaki, Ioannis A. Fotidis*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In this study, strategies for recovery of ammonia-stressed AD reactors were attempted, by addition of preserved bioaugmentation consortium in gel (BioG), fresh consortium in liquid medium (BioL), woodchip biochar (BW), and straw biochar (BS). In comparison to control group with ammonia, effective treatments, i.e., BioG, BioL, BW and BS raised the maximum methane production rate by 77%, 23%, 35%, and 24%, respectively. BW possibly acted as interspecies electrical conduits for Direct Electron Transfer based on conductivity and SEM analysis. BioG facilitated slow release of bioaugmentation inocula from gel into the AD system, which protected them from a direct environmental shock. According to microbial analysis, both BioG, BioL and BW resulted in increased relative abundance of Methanothermobacter thermautotrophicus; and BS induced selective raise of Methanosarcina thermophila. The increase of methanogens via these strategies led to the faster recovery of the AD process.
Original languageEnglish
Article number125329
JournalBioresource Technology
Volume336
Number of pages10
ISSN0960-8524
DOIs
Publication statusPublished - 2021

Keywords

  • Bioaugmentation
  • Biochar
  • Ammonia Inhibition
  • Anaerobic Digestion
  • Selective Enrichment

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