Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster

Søren Langkilde, Malene Schrøder, Thomas Frank, Louise V.T. Shepherd, Sean Conner, Howard V. Davies, Otto A. Meyer, Jürgen Danier, Michael Rychlik, William R. Belknap, Kent F. McCue, Karl-Heinz Engel, Derek Stewart, Ib Knudsen, Morten Poulsen, Morten Poulsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs α-chaconine and α-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of α-solanine: α-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced α-solanine content, and the parental control line (Desirée wild-type) having a traditional α-solanine: α-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different α-solanine: α-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.
Original languageEnglish
JournalRegulatory Toxicology and Pharmacology
Volume64
Issue number1
Pages (from-to)177-185
ISSN0273-2300
DOIs
Publication statusPublished - 2012

Keywords

  • Glycoalkaloid
  • α-Solanine
  • α-Chaconine
  • GM
  • Potato
  • Acetylcholinesterase (AChE)
  • Hamster
  • Toxicity
  • Compositional analyses

Cite this

Langkilde, Søren ; Schrøder, Malene ; Frank, Thomas ; Shepherd, Louise V.T. ; Conner, Sean ; Davies, Howard V. ; Meyer, Otto A. ; Danier, Jürgen ; Rychlik, Michael ; Belknap, William R. ; McCue, Kent F. ; Engel, Karl-Heinz ; Stewart, Derek ; Knudsen, Ib ; Poulsen, Morten ; Poulsen, Morten. / Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster. In: Regulatory Toxicology and Pharmacology. 2012 ; Vol. 64, No. 1. pp. 177-185.
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title = "Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster",
abstract = "Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs α-chaconine and α-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of α-solanine: α-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced α-solanine content, and the parental control line (Desir{\'e}e wild-type) having a traditional α-solanine: α-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60{\%} freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different α-solanine: α-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.",
keywords = "Glycoalkaloid, α-Solanine, α-Chaconine, GM, Potato, Acetylcholinesterase (AChE), Hamster, Toxicity, Compositional analyses",
author = "S{\o}ren Langkilde and Malene Schr{\o}der and Thomas Frank and Shepherd, {Louise V.T.} and Sean Conner and Davies, {Howard V.} and Meyer, {Otto A.} and J{\"u}rgen Danier and Michael Rychlik and Belknap, {William R.} and McCue, {Kent F.} and Karl-Heinz Engel and Derek Stewart and Ib Knudsen and Morten Poulsen and Morten Poulsen",
year = "2012",
doi = "10.1016/j.yrtph.2012.06.018",
language = "English",
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pages = "177--185",
journal = "Regulatory Toxicology and Pharmacology",
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Langkilde, S, Schrøder, M, Frank, T, Shepherd, LVT, Conner, S, Davies, HV, Meyer, OA, Danier, J, Rychlik, M, Belknap, WR, McCue, KF, Engel, K-H, Stewart, D, Knudsen, I, Poulsen, M & Poulsen, M 2012, 'Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster', Regulatory Toxicology and Pharmacology, vol. 64, no. 1, pp. 177-185. https://doi.org/10.1016/j.yrtph.2012.06.018

Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster. / Langkilde, Søren; Schrøder, Malene; Frank, Thomas; Shepherd, Louise V.T.; Conner, Sean; Davies, Howard V.; Meyer, Otto A.; Danier, Jürgen; Rychlik, Michael; Belknap, William R.; McCue, Kent F.; Engel, Karl-Heinz; Stewart, Derek; Knudsen, Ib; Poulsen, Morten; Poulsen, Morten.

In: Regulatory Toxicology and Pharmacology, Vol. 64, No. 1, 2012, p. 177-185.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Compositional and toxicological analysis of a GM potato line with reduced α-solanine content – A 90-day feeding study in the Syrian Golden hamster

AU - Langkilde, Søren

AU - Schrøder, Malene

AU - Frank, Thomas

AU - Shepherd, Louise V.T.

AU - Conner, Sean

AU - Davies, Howard V.

AU - Meyer, Otto A.

AU - Danier, Jürgen

AU - Rychlik, Michael

AU - Belknap, William R.

AU - McCue, Kent F.

AU - Engel, Karl-Heinz

AU - Stewart, Derek

AU - Knudsen, Ib

AU - Poulsen, Morten

AU - Poulsen, Morten

PY - 2012

Y1 - 2012

N2 - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs α-chaconine and α-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of α-solanine: α-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced α-solanine content, and the parental control line (Desirée wild-type) having a traditional α-solanine: α-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different α-solanine: α-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.

AB - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs α-chaconine and α-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of α-solanine: α-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced α-solanine content, and the parental control line (Desirée wild-type) having a traditional α-solanine: α-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different α-solanine: α-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.

KW - Glycoalkaloid

KW - α-Solanine

KW - α-Chaconine

KW - GM

KW - Potato

KW - Acetylcholinesterase (AChE)

KW - Hamster

KW - Toxicity

KW - Compositional analyses

U2 - 10.1016/j.yrtph.2012.06.018

DO - 10.1016/j.yrtph.2012.06.018

M3 - Journal article

VL - 64

SP - 177

EP - 185

JO - Regulatory Toxicology and Pharmacology

JF - Regulatory Toxicology and Pharmacology

SN - 0273-2300

IS - 1

ER -