Complete structures of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides

Andrew Preston, Bent Petersen, Jens Øllgaard Duus, Joanna Kubler-Kielb, Gil Ben-Menachem, Jianjun Li, Evgeny Vinogradov

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The structures of the lipopolysaccharide (LPS) core and O antigen of Bordetella bronchiseptica and Bordetella parapertussis are known, but how these two regions are linked to each other had not been determined. We have studied LPS from several strains of these microorganisms to determine the complete carbohydrate structure of the LPS. LPS was analyzed using different chemical degradations, NMR spectroscopy, and mass spectrometry. This identified a novel pentasaccharide fragment that links the O chain to the core in all the LPS studied. In addition, although the O chain of these bacteria was reported as a homopolymer of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid, we discovered that the polymer contains several amidated uronic acids, the number of which varies between strains. These new data describe the complete structure of the LPS carbohydrate backbone for both Bordetella species and help to explain the complex genetics of LPS biosynthesis in these bacteria.
Original languageEnglish
JournalJournal of Biological Chemistry
Volume281
Issue number26
Pages (from-to)18135-18144
Number of pages10
ISSN0021-9258
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Bordetella bronchiseptica
  • Bordetella parapertussis
  • Carbohydrate Sequence
  • Lipopolysaccharides
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular
  • Species Specificity
  • Spectrometry, Mass, Electrospray Ionization
  • Biochemistry
  • Biopolymers
  • Biosynthesis
  • Carbohydrates
  • Microorganisms
  • Molecular structure
  • Polysaccharides
  • carbohydrate
  • galacturonic acid
  • lipopolysaccharide
  • pentasaccharide
  • phenylacetic acid derivative
  • polymer
  • amidation
  • article
  • bacterial strain
  • controlled study
  • electrospray mass spectrometry
  • microorganism
  • nonhuman
  • nuclear magnetic resonance spectroscopy
  • priority journal
  • protein analysis
  • protein degradation
  • protein determination
  • protein structure
  • Bacteria (microorganisms)
  • Bordetella
  • Bordetella species
  • Carbohydrate backbone
  • Homopolymer
  • Uronic acids
  • T
  • X
  • BIOCHEMISTRY
  • RESPIRATORY-TRACT INFECTION
  • BIOSYNTHESIS LOCUS
  • O-ANTIGEN
  • PERTUSSIS
  • POLYSACCHARIDES
  • HOST
  • WLB
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Alcaligenaceae [06502] Bordetella bronchiseptica species strain-10580, strain-BAA-588 Bordetella parapertussis species strain-15311, strain-BAA-587, strain-15989
  • 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid
  • lipopolysaccharide LPS degradation, biosynthesis
  • O antigen
  • uronic acids
  • 10060, Biochemistry studies - General
  • 10066, Biochemistry studies - Lipids
  • 10068, Biochemistry studies - Carbohydrates
  • 31000, Physiology and biochemistry of bacteria
  • mass spectrometry laboratory techniques, spectrum analysis techniques
  • NMR spectroscopy laboratory techniques, spectrum analysis techniques
  • Biochemistry and Molecular Biophysics

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