Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes

Thomas Bruun Rasmussen; I. Reimann; Åse Uttenthal; M. Beer

Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes

Thomas Bruun Rasmussen
, I. Reimann
, Åse Uttenthal
, M. Beer

Friedrich-Loeffler-Institute

Research output: Contribution to conference › Conference abstract for conference › Research › peer-review

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Abstract

Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.

Original language	English
Publication date	2011
Publication status	Published - 2011
Event	Workshop on Laboratory Diagnosis of African and Classical Swine Fever - Lipica, Slovenia Duration: 17 May 2011 → 22 May 2011

Workshop

Workshop	Workshop on Laboratory Diagnosis of African and Classical Swine Fever
Country/Territory	Slovenia
City	Lipica
Period	17/05/2011 → 22/05/2011

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title = "Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes",

abstract = "Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.",

author = "Rasmussen, \{Thomas Bruun\} and I. Reimann and {\AA}se Uttenthal and M. Beer",

year = "2011",

language = "English",

note = "Workshop on Laboratory Diagnosis of African and Classical Swine Fever ; Conference date: 17-05-2011 Through 22-05-2011",

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Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes. / Rasmussen, Thomas Bruun; Reimann, I.; Uttenthal, Åse et al.
2011. Abstract from Workshop on Laboratory Diagnosis of African and Classical Swine Fever, Lipica, Slovenia.

Research output: Contribution to conference › Conference abstract for conference › Research › peer-review

TY - ABST

T1 - Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes

AU - Rasmussen, Thomas Bruun

AU - Reimann, I.

AU - Uttenthal, Åse

AU - Beer, M.

PY - 2011

Y1 - 2011

N2 - Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.

AB - Complete genome amplification of viral RNA provides a new tool for the generation of modified pestiviruses. We have used our full-genome amplification strategy for generation of amplicons representing complete genomes of classical swine fever virus. The amplicons were cloned directly into a stable single-copy bacterial artificial chromosome (BAC) generating full-length pestivirus DNAs from which infectious RNA transcripts could be also derived. Our strategy allows construction of stable infectious BAC DNAs from a single full-length PCR product.

M3 - Conference abstract for conference

T2 - Workshop on Laboratory Diagnosis of African and Classical Swine Fever

Y2 - 17 May 2011 through 22 May 2011

ER -

Complete Genomes of Classical Swine Fever Virus Cloned into Bacterial Artificial Chromosomes

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Workshop

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